Department of Medical Physics, University of Wisconsin-Madison, Room 7137, 1111 Highland Ave, Madison, WI 53705-2275, USA.
Small. 2011 Dec 2;7(23):3261-9. doi: 10.1002/smll.201100597. Epub 2011 Sep 20.
Raman spectroscopy has been explored for various biomedical applications (e.g., cancer diagnosis) because it can provide detailed information on the chemical composition of cells and tissues. For imaging applications, several variations of Raman spectroscopy have been developed to enhance its sensitivity. To date, a wide variety of molecular targets and biological events have been investigated using surface-enhanced Raman scattering (SERS)-active nanoparticles. The superb multiplexing capability of SERS-based Raman imaging, already successfully demonstrated in live animals, can be extremely powerful in future research where different agents can be attached to different Raman tags to enable the simultaneous interrogation of multiple biological events. Over the last several years, molecular imaging with SERS-active nanoparticles has advanced significantly and many pivotal proof-of-principle experiments have been successfully carried out. It is expected that SERS-based imaging will continue to be a dynamic research field over the next decade.
拉曼光谱在各种生物医学应用中得到了探索（例如癌症诊断），因为它可以提供有关细胞和组织化学成分的详细信息。对于成像应用，已经开发出几种拉曼光谱变体来提高其灵敏度。迄今为止，已经使用表面增强拉曼散射（SERS）活性纳米粒子研究了广泛的分子靶标和生物事件。SERS 基拉曼成像的出色复用能力已经在活体动物中得到了成功证明，在未来的研究中，它将非常强大，不同的试剂可以附着到不同的拉曼标签上，从而可以同时询问多个生物事件。在过去的几年中，SERS 活性纳米粒子的分子成像有了显著的进展，并且已经成功地进行了许多关键的原理验证实验。预计在未来十年中，基于 SERS 的成像将继续成为一个充满活力的研究领域。
