State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Key Laboratory of Application of Pluripotent Stem Cells in Heart Regeneration, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Stem Cell Res. 2022 Jul;62:102804. doi: 10.1016/j.scr.2022.102804. Epub 2022 May 4.
T-Box Transcription Factor 18 is a member of the T-box family, encoding TBX18 protein. As a transcriptional repressor, it related to developmental processes of a majority of tissues and organs and plays crucial part in the embryonic development of sinoatrial node. Using an episomal vector-based CRISPR/Cas9 system, we have established a homozygous TBX18 knockout (TBX18-KO) human embryonic stem cell (hESC) line. This newly TBX18 hESC line display normal pluripotency, morphology, karyotype and trilineage differentiating capacity. This cell line may provide a powerful tool to investigate the role of TBX18 gene in sinoatrial node development in future.
T 盒转录因子 18 是 T 盒家族的成员，编码 TBX18 蛋白。作为转录抑制因子，它与大多数组织和器官的发育过程有关，在窦房结的胚胎发育中起着关键作用。我们使用基于附加体载体的 CRISPR/Cas9 系统，建立了一个纯合的 TBX18 敲除（TBX18-KO）人胚胎干细胞（hESC）系。这个新的 TBX18 hESC 系显示出正常的多能性、形态、核型和三系分化能力。该细胞系可能为未来研究 TBX18 基因在窦房结发育中的作用提供一个有力的工具。
