Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130117, China.
J Hazard Mater. 2022 Jan 5;421:126710. doi: 10.1016/j.jhazmat.2021.126710. Epub 2021 Jul 21.
The particulate matter (PM) in livestock houses, one of the primary sources of atmospheric PM, is not only detrimental to the respiratory health of animals and farmworkers but also poses a threat to the public environment and public health and warrants increased attention. In this study, we investigated the variation in the pulmonary microbiome and metabolome in broiler chickens exposed to PM collected from a broiler house. We examined the pulmonary microbiome and metabolome in broilers, observing that PM induced a visible change in α and β diversity. A total of 66 differential genera, including unclassified_f_Ruminococcaceae and Campylobacter, were associated with pulmonary inflammation. Untargeted metabolomics was utilised to identify 63 differential metabolites induced by PM and correlated with differential bacteria. We observed that PM resulted in injury of the broiler lung and disruption of the microbial community, as well as causing changes in the observed metabolites. These results imply that perturbations to the microbiome and metabolome may play pivotal roles in the mechanism underlying PM-induced broiler lung damage.
鸡舍中的颗粒物（PM）是大气 PM 的主要来源之一，不仅会损害动物和农场工人的呼吸系统健康，还会对公共环境和公共健康构成威胁，值得引起更多关注。在这项研究中，我们研究了暴露于从肉鸡舍收集的 PM 中的肉鸡肺部微生物组和代谢组的变化。我们检查了肉鸡的肺部微生物组和代谢组，观察到 PM 诱导了 α 和 β 多样性的明显变化。共有 66 个差异属，包括未分类的_Ruminococcaceae 和弯曲杆菌，与肺部炎症有关。非靶向代谢组学用于鉴定 63 个由 PM 诱导并与差异细菌相关的差异代谢物。我们观察到 PM 导致肉鸡肺部损伤和微生物群落破坏，并导致观察到的代谢物发生变化。这些结果表明，微生物组和代谢组的扰动可能在 PM 诱导肉鸡肺部损伤的机制中发挥关键作用。
