Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
Analyst. 2021 Sep 7;146(17):5380-5388. doi: 10.1039/d1an00464f. Epub 2021 Aug 2.
A microfluidic chip has been integrated with a capacitive biosensor based on mass-producible three-dimensional (3D) interdigital electrode arrays. To achieve the monitoring of biosensor preparation and cardiac- and periodontitis-related biomarkers, all the processes were detected in a continuously on-site way. Fabrication steps for the microfluidic chip-bonded 3D interdigital capacitor biosensor include gold thiol modification, the activation of EDC/sulfo-NHS, and the bioconjugation of antibodies. Fluorescent characterization and X-ray photoelectron spectroscopy analysis were applied to assess the successful immobilization of the C-reactive protein (CRP) antibody. The experimental results indicate the good specificity and high sensitivity of the microfluidic integrated 3D capacitive biosensor. The limit of detection of the 3D capacitive biosensor for CRP label-free detection was about 1 pg mL. This 3D capacitive biosensor with integrated microfluidics is mass-producible and has achieved the on-site continuous detection of cardiac- and periodontitis-related biomarkers with high performance.
已将微流控芯片与基于可批量生产的三维（3D）叉指电极阵列的电容式生物传感器集成在一起。为了实现对生物传感器制备和与心脏病及牙周炎相关生物标志物的监测，所有过程都以连续现场的方式进行检测。微流控芯片键合 3D 叉指电容器生物传感器的制造步骤包括金硫醇修饰、EDC/sulfo-NHS 的活化和抗体的生物偶联。荧光特性分析和 X 射线光电子能谱分析用于评估 C 反应蛋白（CRP）抗体的成功固定。实验结果表明，微流控集成 3D 电容式生物传感器具有良好的特异性和高灵敏度。用于 CRP 无标记检测的 3D 电容式生物传感器的检测限约为 1 pg mL。这种具有集成微流控的 3D 电容式生物传感器可批量生产，并已实现高性能的与心脏病及牙周炎相关生物标志物的现场连续检测。
