Terahertz Sensing and Imaging Research Team, RIKEN Center for Advanced Photonics, Sendai, Miyagi, Japan.
Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
PLoS One. 2021 Aug 2;16(8):e0248381. doi: 10.1371/journal.pone.0248381. eCollection 2021.
Biological phenomena induced by terahertz (THz) irradiation are described in recent reports, but underlying mechanisms, structural and dynamical change of specific molecules are still unclear. In this paper, we performed time-lapse morphological analysis of human cells and found that THz irradiation halts cell division at cytokinesis. At the end of cytokinesis, the contractile ring, which consists of filamentous actin (F-actin), needs to disappear; however, it remained for 1 hour under THz irradiation. Induction of the functional structures of F-actin was also observed in interphase cells. Similar phenomena were also observed under chemical treatment (jasplakinolide), indicating that THz irradiation assists actin polymerization. We previously reported that THz irradiation enhances the polymerization of purified actin in vitro; our current work shows that it increases cytoplasmic F-actin in vivo. Thus, we identified one of the key biomechanisms affected by THz waves.
最近的报告描述了太赫兹（THz）辐照诱导的生物现象，但特定分子的潜在机制和结构动力学变化仍不清楚。在本文中，我们对人细胞进行了延时形态分析，发现 THz 辐照在细胞分裂末期阻止细胞分裂。在细胞分裂末期，由丝状肌动蛋白（F-actin）组成的收缩环需要消失；然而，在 THz 辐照下，它持续了 1 小时。在有丝分裂细胞中也观察到 F-actin 的功能结构的诱导。类似的现象也在化学处理（jasplakinolide）下观察到，表明 THz 辐照有助于肌动蛋白聚合。我们之前报道过 THz 辐照增强了体外纯化肌动蛋白的聚合；我们目前的工作表明，它增加了细胞浆中的 F-actin。因此，我们确定了 THz 波影响的关键生物力学机制之一。
