School of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, PR China.
Int J Syst Evol Microbiol. 2021 Aug;71(8). doi: 10.1099/ijsem.0.004935.
A Gram-negative bacterium, designated S1-65, was isolated from soil samples collected from a cotton field located in the Xinjiang region of PR China. Results of 16S rRNA gene sequence analysis revealed that strain S1-65 was affiliated to the genus with its closest phylogenetic relatives being '' 35Y (98.4 %), '' SA29-B (98.3 %) and KA5-B (98.3 %). 16S rRNA-directed phylogenetic analysis showed that strain S1-65 formed a unique phylogenetic subclade next to '' SA29-B and KA5-B, suggesting that strain S1-65 should be identified as a member of the genus . Further, substantial differences between the genotypic properties of strain S1-65 and the members of the genus , including average nucleotide identity and digital DNA-DNA hybridization, resolved the taxonomic position of strain S1-65 and suggested its positioning as representing a novel species of the genus . The DNA G+C content of strain S1-65 was 62.5 mol%, based on its draft genome sequence. The predominant respiratory quinone was ubiquinone-8. The main fatty acids were identified as summed feature 3 (Cω6/Cω7), C and iso-C. In addition, its polar lipid profile was composed of aminophospholipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Here, we propose a novel species of the genus : sp. nov. with the type strain S1-65 (=JCM 34287=CGMCC 1.18736).
一株革兰氏阴性菌，命名为 S1-65，从位于中国新疆地区的棉田土壤样本中分离得到。16S rRNA 基因序列分析结果表明，S1-65 菌株与属内亲缘关系最近的菌株为“35Y（98.4%）”、“SA29-B（98.3%）”和“KA5-B（98.3%）”。16S rRNA 系统发育分析显示，S1-65 菌株与“SA29-B”和“KA5-B”形成了一个独特的系统发育分支，表明 S1-65 菌株应被鉴定为属的一个成员。此外，S1-65 菌株的基因型特性与属内成员之间存在实质性差异，包括平均核苷酸同一性和数字 DNA-DNA 杂交，解决了 S1-65 菌株的分类地位，并表明其应被定位为代表属的一个新种。根据其基因组草图，S1-65 菌株的 DNA G+C 含量为 62.5mol%。主要呼吸醌为泛醌-8。主要脂肪酸为 summed feature 3（Cω6/Cω7）、C 和 iso-C。此外，其极性脂谱由胺磷脂、双磷脂酰甘油、磷脂乙醇胺和磷脂酰甘油组成。在此，我们提出了一个新的属种：命名为“35Y 型”（=JCM 34287=CGMCC 1.18736）。
