Herein we report a novel electrochemical sensing chip and a point-of-care device (PoC) for enzyme-free electrochemical detection of urea in human blood. The electrochemical sensing chip was developed by 3-D printing of conductive Ag ink and subsequent electrodeposition of AuNP-rGO nanocomposite. Material characterization of the sensing chip was conducted to find a plausible mechanism for the electrochemical reaction with urea. Subsequently, the response with varying concentrations of urea in solution and human blood samples was tested. High peak response current (~5 times than that of the highest reported value), low impedance, rapid sensor fabrication procedure, high selectivity towards urea, excellent linear response (R = 0.99), high sensitivity of 183 μA mM cm, the fast response indicated by high diffusion coefficient, the limit of detection of 0.1 µM, tested shelf life of more than 6 months and recovery rate of >99% ensured the application of the developed sensor chip towards PoC urea detection test kit. A PoC device housing an electronic circuitry following the principles of linear sweep voltammetry and compatible with a sensing chip was developed. A maximum percentage error of 4.86% and maximum RSD of 3.63% confirmed the use of the PoC device for rapid urea measurements in human blood.