Elancheziyan Mari, Prakasham Karthikeyan, Eswaran Muthusankar, Duraisamy Murugesan, Ganesan Sivarasan, Lee Siew Ling, Ponnusamy Vinoth Kumar
Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung City-807, Taiwan; Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam-602105, India.
Research Center for Precision Environmental Medicine, Kaohsiung Medical University, Kaohsiung City-807, Taiwan.
Environ Res. 2023 Apr 15;223:115403. doi: 10.1016/j.envres.2023.115403. Epub 2023 Feb 6.
The design and development of eco-friendly fabrication of cost-effective electrochemical nonenzymatic biosensors with enhanced sensitivity and selectivity are one of the emerging area in nanomaterial and analytical chemistry. In this aspect, we developed a facile fabrication of tertiary nanocomposite material based on cobalt and polymelamine/nitrogen-doped graphitic porous carbon nanohybrid composite (Co-PM-NDGPC/SPE) for the application as a nonenzymatic electrochemical sensor to quantify glucose in human blood samples. Co-PM-NDGPC/SPE nanocomposite electrode fabrication was achieved using a single-step electrodeposition method under cyclic voltammetry (CV) technique under 1 M NHCl solution at 20 constitutive CV cycles (sweep rate 20 mV/s). Notably, the fabricated nonenzymatic electroactive nanocomposite material exhibited excellent electrocatalytic sensing towards the quantification of glucose in 0.1 M NaOH over a wide concentration range from 0.03 to 1.071 mM with a sensitive limit of detection 7.8 μM. Moreover, the Co-PM-NDGPC nanocomposite electrode with low charge transfer resistance (Rct∼81 Ω) and high ionic diffusion indicates excellent stability, reproducibility, and high sensitivity. The fabricated nanocomposite materials exhibit a commendable sensing response toward glucose molecules present in the blood serum samples recommends its usage in real-time applications.
设计和开发具有更高灵敏度和选择性的、成本效益高的环保型电化学非酶生物传感器是纳米材料和分析化学领域中一个新兴的研究方向。在这方面，我们开发了一种基于钴与聚三聚氰胺/氮掺杂石墨多孔碳纳米杂化复合材料的三元纳米复合材料（Co-PM-NDGPC/SPE）的简便制备方法，用于作为非酶电化学传感器来定量检测人血样本中的葡萄糖。Co-PM-NDGPC/SPE纳米复合电极的制备是在1M NHCl溶液中，于20个本构循环伏安（CV）周期（扫描速率20mV/s）下，采用循环伏安（CV）技术通过单步电沉积法实现的。值得注意的是，制备的非酶电活性纳米复合材料在0.1M NaOH中对葡萄糖定量检测表现出优异的电催化传感性能，检测浓度范围为0.03至1.071mM，检测限低至7.8μM。此外，具有低电荷转移电阻（Rct∼81Ω）和高离子扩散率的Co-PM-NDGPC纳米复合电极表现出优异的稳定性、重现性和高灵敏度。制备的纳米复合材料对血清样本中存在的葡萄糖分子表现出值得称赞的传感响应，这表明其可用于实时应用。
