Annaç Ebru, Uçkun Miraç, Özkaya Ahmet, Yoloğlu Ertan, Pekmez Hıdır, Bulmuş Özgür, Aydın Ali
Department of Histology and Embryology, Faculty of Medicine, Adıyaman University, Adıyaman, Turkey.
Department of Food Engineering, Faculty of Engineering, Adiyaman University, Adiyaman, Turkey.
J Food Biochem. 2022 Apr;46(4):e13881. doi: 10.1111/jfbc.13881. Epub 2021 Aug 4.
The purpose of this study was to investigate the potential side-effects of lead acetate (LA), which is toxic to the nerves, blood and muscles, in the rat brain. The neuroprotective effects of pomegranate juice (PJ) against LA exposure were also observed. The experiment involved 28 male Wistar albino rats aged 12 weeks. These were divided into four groups: Control, PJ, LA and LA+PJ. Stereological techniques were employed to determine hippocampal volume in each rat brain. Biochemical investigations and histopathological examinations were also performed. Analysis demonstrated a significant decrease in hippocampal volume in the LA group compared to the control group (p < .05). The stereology results also indicated that PJ has protective effects when compared with the LA and LA+PJ groups. A significant increase was also determined in malondialdehyde (MDA) levels and glutathione S-transferase (GST) activity in the LA group compared to the control group, in contrast to glutathione (GSH) levels and carboxylesterase (CaE) and acetylcholinesterase (AchE) activities. MDA and GST activity decreased significantly in the LA+PJ group compared to the LA group in contrast to GSH levels and CaE and AchE activities. Histopathological examination revealed a number of degenerative changes in the LA group. Exposure to LA adversely affects the hippocampus on the male rat brain. It might also be suggested that PJ may ameliorate these deleterious effects.
本研究的目的是调查对神经、血液和肌肉有毒性的醋酸铅（LA）在大鼠脑中的潜在副作用。同时也观察了石榴汁（PJ）对暴露于LA的神经保护作用。实验涉及28只12周龄的雄性Wistar白化大鼠。这些大鼠被分为四组：对照组、PJ组、LA组和LA + PJ组。采用体视学技术测定每只大鼠脑内海马体体积。还进行了生化研究和组织病理学检查。分析表明，与对照组相比，LA组的海马体体积显著减小（p <.05）。体视学结果还表明，与LA组和LA + PJ组相比，PJ具有保护作用。与对照组相比，LA组的丙二醛（MDA）水平和谷胱甘肽S - 转移酶（GST）活性显著增加，而谷胱甘肽（GSH）水平、羧酸酯酶（CaE）和乙酰胆碱酯酶（AchE）活性则相反。与LA组相比，LA + PJ组的MDA和GST活性显著降低，而GSH水平、CaE和AchE活性则相反。组织病理学检查显示LA组有许多退行性变化。暴露于LA会对雄性大鼠脑内的海马体产生不利影响。也可能表明PJ可以改善这些有害影响。
