Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi-110062, India.
Molecular Diagnostics, Genetix Biotech Asia, New Delhi-110015, India.
Crit Rev Biomed Eng. 2021;49(1):21-33. doi: 10.1615/CritRevBiomedEng.2021036595.
The outbreak of coronavirus disease 2019 (COVID-19) has resulted in a world-wide crisis. To contain the virus, it is important to find infected individuals and isolate them to stop transmission. Various diagnostic techniques are used to check for infection. With the havoc that severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has created, it is imperative to work on alternative diagnostic techniques that can be used at both point of care with little or no expertise and at mass testing (i.e., when screening). Despite extensive research, to this date no specific effective treatment or cure is available to neutralize this viral infection. Globally, researchers are working to develop effective treatments, and several vaccines have been approved for public use. We found the studies that we explored for this review using appropriate key words for indexing in PubMed and Google Scholar from 2019 to 2020. We compile various techniques that have been used worldwide to diagnose and treat SARS-CoV-2 and discuss novel methods that may be modified for use in diagnosis and treatment. It is crucial to develop a more specific serological test for diagnosis that can rule out the possibility of COVID-19 and be used for mass testing. An affordable, safe, targeted, effective treatment must be developed to cure this disease, which has created a public health emergency of international concern.
2019 年冠状病毒病(COVID-19)的爆发导致了一场全球性危机。为了控制病毒,找到感染者并将其隔离以阻止传播非常重要。目前使用了各种诊断技术来检查感染情况。由于严重急性呼吸综合征冠状病毒 2(SARS-CoV-2)造成的严重破坏,必须研究替代的诊断技术,这些技术可以在护理点使用,无需或几乎无需专业知识,也可以在大规模检测(即筛查)中使用。尽管进行了广泛的研究,但迄今为止,尚无有效的特定治疗方法或药物可中和这种病毒感染。全球研究人员正在努力开发有效的治疗方法,并且已经有几种疫苗获准供公众使用。我们使用 PubMed 和 Google Scholar 中的适当关键字来搜索 2019 年至 2020 年的相关研究,以探索本综述中所涉及的研究。我们汇总了世界各地用于诊断和治疗 SARS-CoV-2 的各种技术,并讨论了可能经过修改后用于诊断和治疗的新方法。开发一种更具体的血清学诊断测试来排除 COVID-19 的可能性并用于大规模检测至关重要。必须开发一种负担得起、安全、有针对性、有效的治疗方法来治愈这种疾病,这种疾病已经造成了国际关注的公共卫生紧急情况。