University College London Institute of Ophthalmology, London EC1V 9EL, UK.
University College London Institute of Ophthalmology, London EC1V 9EL, UK.
Cell Rep. 2021 Aug 3;36(5):109461. doi: 10.1016/j.celrep.2021.109461.
In development, almost all stratified neurons must migrate from their birthplace to the appropriate neural layer. Photoreceptors reside in the most apical layer of the retina, near their place of birth. Whether photoreceptors require migratory events for fine-positioning and/or retention within this layer is not well understood. Here, we show that photoreceptor nuclei of the developing mouse retina cyclically exhibit rapid, dynein-1-dependent translocation toward the apical surface, before moving more slowly in the basal direction, likely due to passive displacement by neighboring retinal nuclei. Attenuating dynein 1 function in rod photoreceptors results in their ectopic basal displacement into the outer plexiform layer and inner nuclear layer. Synapse formation is also compromised in these displaced cells. We propose that repeated, apically directed nuclear translocation events are necessary to ensure retention of post-mitotic photoreceptors within the emerging outer nuclear layer during retinogenesis, which is critical for correct neuronal lamination.
在发育过程中，几乎所有分层神经元都必须从出生地迁移到适当的神经层。光感受器位于视网膜的最顶端层，靠近它们的出生地。光感受器是否需要迁移事件来进行精细定位和/或保留在这个层中还不是很清楚。在这里，我们表明，发育中的小鼠视网膜的光感受器核周期性地表现出快速的、依赖于动力蛋白-1 的向顶端表面的易位，然后再向基底方向缓慢移动，可能是由于相邻的视网膜核的被动移位。减弱视杆细胞中的动力蛋白 1 功能会导致它们异常的基底移位到外丛状层和内核层。在这些移位的细胞中，突触形成也受到了损害。我们提出，重复的、朝向顶端的核易位事件对于确保有丝分裂后光感受器在视网膜发生过程中保留在外核层中是必要的，这对于正确的神经元分层至关重要。
