在活体鼠视网膜外植体中追踪神经元的运动。

Tracking neuronal motility in live murine retinal explants.

机构信息

University College London, Institute of Ophthalmology, London EC1V 9EL, UK.

出版信息

STAR Protoc. 2021 Dec 9;2(4):101008. doi: 10.1016/j.xpro.2021.101008. eCollection 2021 Dec 17.

DOI:10.1016/j.xpro.2021.101008

PMID:34917982

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8666713/

Abstract

The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated data handling are provided. For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).

摘要

发育中的视网膜经历涉及环绕细胞的显著内视网膜运动的动态组织变化。在这里，我们提供了一个在活体离体鼠视网膜中跟踪视网膜细胞运动的方案。虽然最初应用于杆状和锥状光感受器，但该策略适用于鼠视网膜和其他类似实验性视网膜模型中的任何荧光标记细胞。仔细的组织处理对于成功获取高质量的活体成像数据至关重要。进一步提供了用于半自动数据处理的说明。有关该方案使用和执行的完整详细信息，请参阅 Aghaizu 等人。（2021 年）。