嗜热菌中细胞壁相关直接细胞外电子转移的评估

Evaluation of cell wall-associated direct extracellular electron transfer in thermophilic sp.

作者信息

Gurumurthy Dummi Mahadevan, Bilal Muhammad, Nadda Ashok Kumar, Reddy Vaddi Damodara, Saratale Ganesh Dattatraya, Guzik Urszula, Ferreira Luiz Fernando Romanholo, Gupta Sanjay Kumar, Savanur Mohammed Azharuddin, Mulla Sikandar I

机构信息

Department of Biotechnology, GM Institute of Technology, Davangere, Karnataka 577006 India.

School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an, China.

出版信息

3 Biotech. 2021 Aug;11(8):383. doi: 10.1007/s13205-021-02917-2. Epub 2021 Jul 27.

DOI:10.1007/s13205-021-02917-2

PMID:34350088

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8316523/

Abstract

UNLABELLED

In this study, a cell wall-associated extracellular electron transfer (EET) was determined in the thermophilic sp. to utilize iron as a terminal electron acceptor. The direct extracellular transfer of its electrons was primarily linked to the cell wall cytochrome-c and diffusible redox mediators like flavins during the anoxic condition. Based on the azo dye decolouration and protein film voltammetry, it was revealed that, in the absence of surface polysaccharide and diffusible mediators, the cell wall-associated EET pathway was likely to be a favorable mechanism in sp. Since the permeability of such redox molecule is primarily limited to the cell wall, the electron transfer occurs by direct contact with cell wall-associated cytochrome and final electron acceptor. Furthermore, transfer of electrons with the help of redox shuttling molecules like riboflavin from cytochrome to cells, vice versa indicates that sp. has adopted this unique pathway during an anoxic environment for its respiration.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-021-02917-2.

摘要

未标注

在本研究中，测定了嗜热菌中的一种细胞壁相关细胞外电子转移（EET），以利用铁作为末端电子受体。在缺氧条件下，其电子的直接细胞外转移主要与细胞壁细胞色素c和黄素等可扩散氧化还原介质有关。基于偶氮染料脱色和蛋白质膜伏安法，结果表明，在没有表面多糖和可扩散介质的情况下，细胞壁相关的EET途径可能是嗜热菌中的一种有利机制。由于此类氧化还原分子的通透性主要限于细胞壁，电子转移通过与细胞壁相关细胞色素和最终电子受体直接接触而发生。此外，借助核黄素等氧化还原穿梭分子将电子从细胞色素转移到细胞，反之亦然，这表明嗜热菌在缺氧环境中采用了这种独特的途径进行呼吸作用。