利用细胞脂肪酸和中性单糖的气相色谱分析鉴定乳酸杆菌。
Gas chromatography analysis of cellular fatty acids and neutral monosaccharides in the identification of lactobacilli.
作者信息
Rizzo A F, Korkeala H, Mononen I
机构信息
Department of Chemistry, National Veterinary Institute, Helsinki, Finland.
出版信息
Appl Environ Microbiol. 1987 Dec;53(12):2883-8. doi: 10.1128/aem.53.12.2883-2888.1987.
Abstract
Cellular fatty acids and monosaccharides in a group of 14 lactobacilli were analyzed by gas chromatography and the identity of the components was confirmed by gas chromatography-mass spectrometry. From the same bacterial sample, both monosaccharides and fatty acids were liberated by methanolysis, and in certain experiments, fatty acids alone were released by basic hydrolysis. The results indicate that basic hydrolysis gave more comprehensive information about the fatty acids, but the analysis of monosaccharides was found to be much more useful in distinguishing between different species of lactobacilli. The method described allowed differentiation of 11 of 14 Lactobacillus species, and even single colonies isolated from agar plates could be used for analysis without subculturing.
摘要
通过气相色谱法分析了一组14株乳酸杆菌中的细胞脂肪酸和单糖，并通过气相色谱-质谱联用仪确认了各成分的身份。从同一细菌样本中，单糖和脂肪酸都通过甲醇解作用释放出来，在某些实验中，仅脂肪酸通过碱性水解释放出来。结果表明，碱性水解能提供有关脂肪酸更全面的信息，但发现单糖分析在区分不同种类的乳酸杆菌方面更有用。所描述的方法能够区分14种乳酸杆菌中的11种，甚至从琼脂平板上分离出的单个菌落无需传代培养即可用于分析。
相似文献
1
Gas chromatography analysis of cellular fatty acids and neutral monosaccharides in the identification of lactobacilli.利用细胞脂肪酸和中性单糖的气相色谱分析鉴定乳酸杆菌。
Appl Environ Microbiol. 1987 Dec;53(12):2883-8. doi: 10.1128/aem.53.12.2883-2888.1987.
2
Fatty acid composition of Bifidobacterium and Lactobacillus strains.双歧杆菌和乳酸杆菌菌株的脂肪酸组成。
J Bacteriol. 1971 Nov;108(2):861-7. doi: 10.1128/jb.108.2.861-867.1971.
3
Gas chromatography of bacterial whole cell methanolysates; IV. A procedure for fractionation and identification of fatty acids and monosaccharides of cellular structures.细菌全细胞甲醇解产物的气相色谱法；IV. 细胞结构中脂肪酸和单糖的分离与鉴定方法
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):753-66.
4
5
Lipids of alcoholophilic lactobacilli. II. Occurrence of polar lipids with unusually long acyl chains in Lactobacillus heterohiochii.嗜酒精乳杆菌的脂质。II. 嗜异源乳杆菌中具有异常长酰基链的极性脂质的存在情况。
Biochim Biophys Acta. 1974 Nov 18;369(2):146-55. doi: 10.1016/0005-2760(74)90248-3.
6
Relationship of cellular fatty acid composition to survival of Lactobacillus bulgaricus in liquid nitrogen.保加利亚乳杆菌细胞脂肪酸组成与在液氮中存活率的关系
Appl Microbiol. 1974 Apr;27(4):738-43. doi: 10.1128/am.27.4.738-743.1974.
7
Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids.通过细胞脂肪酸气-液相色谱法进行细菌自动鉴定的最佳数据处理程序
J Clin Microbiol. 1988 Sep;26(9):1745-53. doi: 10.1128/jcm.26.9.1745-1753.1988.
8
Gas chromatography and mass spectrometry of disaccharides from glycoproteins.
J Biochem Biophys Methods. 1979 Aug;1(4):227-35. doi: 10.1016/0165-022x(79)90009-5.
9
Cellular fatty acids analysis in the identification of lactic acid bacteria.
Int J Food Microbiol. 1994 Dec;24(1-2):315-9. doi: 10.1016/0168-1605(94)90129-5.
10
Quantitative cleavage of the N-glycosidic bond under the normal conditions of methanolysis used for the analysis of glycoprotein monosaccharides.在用于分析糖蛋白单糖的甲醇解正常条件下，N-糖苷键的定量裂解。
Anal Biochem. 1999 Feb 15;267(2):300-8. doi: 10.1006/abio.1998.2967.
引用本文的文献
1
Immunostimulatory activity of lipoteichoic acid with three fatty acid residues derived from JCM 15950.三脂基化脂磷壁酸源自 JCM 15950 的免疫刺激活性。
Appl Environ Microbiol. 2024 Oct 23;90(10):e0119724. doi: 10.1128/aem.01197-24. Epub 2024 Sep 6.
2
Preparation and Structural Analysis of Lipoteichoic Acid on Cell Membranes Derived from Lactic Acid Bacteria.从乳酸菌中提取的细胞膜脂磷壁酸的制备与结构分析。
Methods Mol Biol. 2024;2851:39-60. doi: 10.1007/978-1-0716-4096-8_4.
3
Tween 80™-induced changes in fatty acid profile of selected mesophilic lactobacilli.吐温 80™诱导的部分嗜温乳杆菌脂肪酸组成变化。
Acta Biochim Pol. 2024 Jul 4;71:13014. doi: 10.3389/abp.2024.13014. eCollection 2024.
4
Comparison of Phenotypic and Genotypic Methods Used for the Species Identification of Lactobacillus NP51 and Development of a Strain-Specific PCR Assay.用于乳酸杆菌NP51菌种鉴定的表型和基因型方法比较及菌株特异性PCR检测方法的建立
Probiotics Antimicrob Proteins. 2010 Dec;2(4):274-83. doi: 10.1007/s12602-010-9057-0.
5
Sources of variation in the analysis of trichothecenes in cereals by gas chromatography-mass spectrometry.谷物中玉米赤霉烯酮的气相色谱-质谱分析中的变异来源。
Mycotoxin Res. 2001 Jun;17(2):68-87. doi: 10.1007/BF02946130.
6
Physiological and transcriptional response of Lactobacillus casei ATCC 334 to acid stress.干酪乳杆菌 ATCC 334 对酸胁迫的生理和转录响应。
J Bacteriol. 2010 May;192(9):2445-58. doi: 10.1128/JB.01618-09. Epub 2010 Mar 5.
7
Antifungal 3-hydroxy fatty acids from Lactobacillus plantarum MiLAB 14.来自植物乳杆菌MiLAB 14的抗真菌3-羟基脂肪酸
Appl Environ Microbiol. 2003 Dec;69(12):7554-7. doi: 10.1128/AEM.69.12.7554-7557.2003.
8
Stability of cyclopropane and conjugated linoleic acids during fatty acid quantification in lactic acid bacteria.乳酸菌脂肪酸定量分析过程中环丙烷脂肪酸和共轭亚油酸的稳定性
Lipids. 1999 Oct;34(10):1107-15. doi: 10.1007/s11745-999-0462-8.
9
Optimal data processing procedure for automatic bacterial identification by gas-liquid chromatography of cellular fatty acids.通过细胞脂肪酸气-液相色谱法进行细菌自动鉴定的最佳数据处理程序
J Clin Microbiol. 1988 Sep;26(9):1745-53. doi: 10.1128/jcm.26.9.1745-1753.1988.
10
Carbohydrate patterns, cellular lipoquinones, fatty acids and phospholipids of the genus Pasteurella sensu stricto.狭义巴斯德氏菌属的碳水化合物模式、细胞脂醌、脂肪酸和磷脂。
Med Microbiol Immunol. 1991;180(2):79-92. doi: 10.1007/BF00193849.
本文引用的文献
1
EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI.温度对大肠杆菌脂肪酸组成的影响
J Bacteriol. 1962 Dec;84(6):1260-7. doi: 10.1128/jb.84.6.1260-1267.1962.
2
The anaerobically cultured cecal flora of adult fowls that protects chickens from Salmonella infections.成年家禽经厌氧培养的盲肠菌群可保护鸡免受沙门氏菌感染。
Acta Pathol Microbiol Scand B. 1981 Apr;89(2):109-16. doi: 10.1111/j.1699-0463.1981.tb00161_89b.x.
3
Rapid gas-chromatographic method for identification of metabolic products of anaerobic bacteria.用于鉴定厌氧细菌代谢产物的快速气相色谱法。
J Clin Microbiol. 1980 Apr;11(4):418-21. doi: 10.1128/jcm.11.4.418-421.1980.
4
The effects of nalidixic acid on respiratory activity of asynchronous and synchronous cultures of Alcaligenes eutrophus.
J Gen Microbiol. 1983 Jan;129(1):1-5. doi: 10.1099/00221287-129-1-1.
5
Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria.酸水解和碱水解对细菌中羟基脂肪酸和环丙烷脂肪酸影响的比较。
J Clin Microbiol. 1983 Dec;18(6):1370-7. doi: 10.1128/jcm.18.6.1370-1377.1983.
6
Effect of substrate on fatty acid production in Nocardia asteroides.底物对星形诺卡氏菌脂肪酸产生的影响。
Can J Microbiol. 1970 Apr;16(4):213-7. doi: 10.1139/m70-039.
7
Some factors affecting cyclopropane acid formation in Escherichia coli.一些影响大肠杆菌中环丙烷酸形成的因素。
Biochem J. 1965 Sep;96(3):771-6. doi: 10.1042/bj0960771.
8
An assessment of methanolysis and other factors used in the analysis of carbohydrate-containing materials.对甲醇解及用于含碳水化合物材料分析的其他因素的评估。
Biochem J. 1971 Dec;125(4):1009-18. doi: 10.1042/bj1251009.
9
Fatty acid composition of Bifidobacterium and Lactobacillus strains.双歧杆菌和乳酸杆菌菌株的脂肪酸组成。
J Bacteriol. 1971 Nov;108(2):861-7. doi: 10.1128/jb.108.2.861-867.1971.
10
Changes in the fatty acid composition of Staphylococcus aureus under various cultural conditions.
Acta Microbiol Acad Sci Hung. 1967;14(3):293-8.
Zotero 插件