Laboratório de Imunobiológicos e Virologia Animal, Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, MG, 36570-000, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Av. Peter Henry Rolfs, s/n, Viçosa, MG, 36570-000, Brazil.
Braz J Microbiol. 2021 Dec;52(4):2529-2534. doi: 10.1007/s42770-021-00546-8. Epub 2021 Aug 6.
Bovine alphaherpesvirus 1 (BoHV-1) is a pathogen causing respiratory and reproductive clinical signs in cattle. Infected animals may develop rhinotracheitis, vulvovaginitis, balanoposthitis, and abortion. Viral latency is generally established in neuronal ganglia simultaneously to a decrease in both genes or genome expression and viral replication. Under stressful conditions, infection is reactivated leading to viral replication and the manifestation of clinical signs. In this study, we evaluated both viral reactivation and apoptosis in trigeminal ganglia cells as BoHV-1 progressed from the latent to the acute phase of infection after dexamethasone administration in experimentally infected calves. To test ganglia cell death as a consequence of BoHV-1 infection, we stained the BoHV-1 samples with TUNEL after the viral shedding by the calves. RT-qPCR of apoptotic genes was also performed, showing the upregulation of the caspase 8 gene in the trigeminal ganglia from cattle experimentally infected with BoHV-1. These results showed the occurrence of apoptosis in ganglion cells of calves infected by BoHV-1.
牛α疱疹病毒 1（BoHV-1）是一种病原体，可引起牛的呼吸道和生殖系统临床症状。感染动物可能会发展为鼻炎、阴道炎、龟头炎和流产。病毒潜伏期通常与基因或基因组表达和病毒复制的减少同时在神经元神经节中建立。在应激条件下，感染会被重新激活，导致病毒复制和临床症状的表现。在这项研究中，我们评估了在实验感染的犊牛中，地塞米松给药后，BoHV-1 从潜伏感染到急性感染阶段的进展过程中，三叉神经节细胞中的病毒再激活和细胞凋亡。为了检测 BoHV-1 感染导致的神经节细胞死亡，我们在犊牛排出病毒后用 TUNEL 对 BoHV-1 样本进行了染色。对凋亡基因的 RT-qPCR 也表明，感染 BoHV-1 的牛的三叉神经节中 caspase 8 基因的上调。这些结果表明，BoHV-1 感染的犊牛的神经节细胞中发生了细胞凋亡。
