LAQV@REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.
Chemistry. 2021 Oct 7;27(56):13998-14006. doi: 10.1002/chem.202101724. Epub 2021 Sep 8.
To protect their intracellular proteins, extremophile microorganisms synthesize molecules called compatible solutes. These molecules are the result of the attachment of a small negatively charged molecule to a sugar molecule. It has been found that these molecules, not only protect the microorganism against osmotic stress but also against other extreme conditions. They can also confer protection against extreme conditions to isolated enzymes from different organisms making them an exciting prospect for potential biotechnological applications. One of the most widespread compatible solute in hyperthermophile organisms is the molecule 2-O-α-D-mannosyl-D-glycerate (MG). In addition to confer protection to proteins against extreme conditions, MG was found to prevent Alzheimer's β-amyloid aggregation and reduce α-synuclein fibril formation in Parkinson's disease. In this work we studied, using computational methods, the catalytic mechanism of the synthesis of MG by the enzyme mannosylglycerate synthase (MGS) from the thermophilic bacteria Rhodothermus marinus.
为了保护其细胞内蛋白质，极端微生物合成了称为相容性溶质的分子。这些分子是将一个小带负电荷的分子附着到糖分子上的结果。已经发现，这些分子不仅可以保护微生物免受渗透胁迫，还可以免受其他极端条件的影响。它们还可以赋予来自不同生物体的分离酶对抗极端条件的保护，这使得它们成为潜在生物技术应用的令人兴奋的前景。在高温微生物中最广泛的相容溶质之一是 2-O-α-D-甘露糖基-D-甘油酸（MG）分子。除了保护蛋白质免受极端条件的影响外，MG 还被发现可以阻止阿尔茨海默氏症β-淀粉样蛋白聚集，并减少帕金森氏病中α-突触核蛋白纤维的形成。在这项工作中，我们使用计算方法研究了来自嗜热细菌 Rhodothermus marinus 的甘露糖甘油酸合酶（MGS）合成 MG 的催化机制。
