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某些商业苹果品种提取物在两种人结肠直肠癌和胶质母细胞瘤细胞系中的抗氧化、抗炎及细胞毒性活性

Antioxidant, Anti-Inflammatory, and Cytotoxic Activity of Extracts from Some Commercial Apple Cultivars in Two Colorectal and Glioblastoma Human Cell Lines.

作者信息

Butkeviciute Aurita, Petrikaite Vilma, Jurgaityte Vidmante, Liaudanskas Mindaugas, Janulis Valdimaras

机构信息

Department of Pharmacognosy, Lithuanian University of Health Sciences, Sukileliu av. 13, LT-50162 Kaunas, Lithuania.

Laboratory of Drug Targets Histopathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu av. 13, LT-50162 Kaunas, Lithuania.

出版信息

Antioxidants (Basel). 2021 Jul 8;10(7):1098. doi: 10.3390/antiox10071098.

DOI:10.3390/antiox10071098
PMID:34356331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8301036/
Abstract

Cancer initiation and development are closely related to oxidative stress and chronic inflammation. The aim of this study was to evaluate apple extracts and individual tritepenes antioxidant, anti-inflammatory, and cytotoxic activities. Dry extracts of apple were analyzed by HPLC-PDA. A hyaluronidase inhibition assay was selected to determine the anti-inflammatory effect. Cytotoxic activities against human colon adenocarcinoma cell line (HT-29) and human glioblastoma cell line (U-87) were determined using MTT, cell colony formation, and spheroid growth assays. Radical scavenging and reducing activities were evaluated using DPPH, ABTS, FRAP, and CUPRAC assays, respectively. The apple extracts inhibited hyaluronidase from 26.38 ± 4.4% to 35.05 ± 3.8%. The AAW extract possessed the strongest cytotoxic activity (EC varied from 113.3 ± 11.11 µg/mL to 119.7 ± 4.0 µg/mL). The AEW extract had four and five times stronger antiradical activity when determined by ABTS and DPPH, and two and eight times stronger reducing activity when evaluated by CUPRAC and FRAP, respectively. Understanding the mechanisms of apple extracts and individual triterpenes as hyaluronidase inhibitors and antioxidants related in cancer development may be a benefit to future study in vivo, as well as cancer prognosis or the development of new, innovative food supplements, which could be used for chronic disease prevention.

摘要

癌症的起始和发展与氧化应激和慢性炎症密切相关。本研究的目的是评估苹果提取物和单个三萜类化合物的抗氧化、抗炎和细胞毒性活性。采用高效液相色谱-光电二极管阵列检测器（HPLC-PDA）分析苹果干提取物。选择透明质酸酶抑制试验来确定抗炎效果。使用MTT、细胞集落形成和球体生长试验来测定对人结肠腺癌细胞系（HT-29）和人胶质母细胞瘤细胞系（U-87）的细胞毒性活性。分别使用DPPH、ABTS、FRAP和CUPRAC试验评估自由基清除和还原活性。苹果提取物对透明质酸酶的抑制率从26.38±4.4%到35.05±3.8%。AAW提取物具有最强的细胞毒性活性（EC值在113.3±11.11μg/mL至119.7±4.0μg/mL之间变化）。通过ABTS和DPPH测定时，AEW提取物的抗自由基活性分别强四倍和五倍；通过CUPRAC和FRAP评估时，其还原活性分别强两倍和八倍。了解苹果提取物和单个三萜类化合物作为透明质酸酶抑制剂和与癌症发展相关的抗氧化剂的作用机制，可能有利于未来的体内研究以及癌症预后或新型创新食品补充剂的开发，这些食品补充剂可用于预防慢性疾病。

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