Liaudanskas Mindaugas, Žvikas Vaidotas, Petrikaitė Vilma
Faculty of Pharmacy, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania.
Institute of Cardiology, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania.
Antioxidants (Basel). 2021 Jul 12;10(7):1115. doi: 10.3390/antiox10071115.
In modern society, cancer is one of the most relevant medical problems. It is important to search for promising plant raw materials whose extracts have strong antioxidant and anticancer effects. The aim of this study was to determine the composition of phenolic compounds in plant extracts, to evaluate their antioxidant and anticancer activity, and to find the correlations between those activities. Extracts of calendula, sage, bearberry, eucalyptus, yarrow, and apple were selected for the study. The phenolic compounds of these extracts were determined by the UPLC-ESI-MS/MS method and the antioxidant activity was evaluated in vitro by four different UV-VIS spectrophotometric methods (ABTS, DPPH, CUPRAC, FRAP). The anticancer activity of extracts was tested against melanoma IGR39, glioblastoma U-87, and triple-negative breast cancer MDA-MB-231 cell lines in vitro by MTT assay. The highest content of identified and quantified phenolic compounds was found in sage leaf extract and the lowest in ethanol eucalyptus leaf extract. The highest antioxidant activity was determined by all applied methods for the acetone eucalyptus leaf extract. The majority of extracts were mostly active against the melanoma IGR39 cell line, and possessed the lowest activity against the glioblastoma U-87 cell line. Acetone extract of eucalyptus leaf samples exhibited the highest anticancer activity against all tested cell lines. Strong and reliable correlation has been found between antioxidant and anticancer activity in breast cancer and glioblastoma cell lines, especially when evaluating antioxidant activity by the FRAP method.
在现代社会，癌症是最相关的医学问题之一。寻找有前景的植物原料非常重要，其提取物具有强大的抗氧化和抗癌作用。本研究的目的是确定植物提取物中酚类化合物的组成，评估其抗氧化和抗癌活性，并找出这些活性之间的相关性。选择了金盏花、鼠尾草、熊果、桉树、蓍草和苹果的提取物进行研究。这些提取物中的酚类化合物通过超高效液相色谱-电喷雾串联质谱法（UPLC-ESI-MS/MS）测定，抗氧化活性通过四种不同的紫外可见分光光度法（ABTS、DPPH、CUPRAC、FRAP）在体外进行评估。提取物的抗癌活性通过MTT法在体外针对黑色素瘤IGR39、胶质母细胞瘤U-87和三阴性乳腺癌MDA-MB-231细胞系进行测试。在鼠尾草叶提取物中发现已鉴定和定量的酚类化合物含量最高，而在乙醇桉树叶提取物中含量最低。所有应用方法测定丙酮桉树叶提取物的抗氧化活性最高。大多数提取物对黑色素瘤IGR39细胞系的活性最强，对胶质母细胞瘤U-87细胞系的活性最低。桉树叶样品的丙酮提取物对所有测试细胞系均表现出最高的抗癌活性。在乳腺癌和胶质母细胞瘤细胞系中，抗氧化和抗癌活性之间发现了强而可靠的相关性，尤其是在用FRAP法评估抗氧化活性时。
