Aldawsari Mohammed F, Khafagy El-Sayed, Saqr Ahmed Al, Alalaiwe Ahmed, Abbas Hisham A, Shaldam Moataz A, Hegazy Wael A H, Goda Reham M
Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia.
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Antibiotics (Basel). 2021 Jul 17;10(7):871. doi: 10.3390/antibiotics10070871.
The bacterial resistance development due to the incessant administration of antibiotics has led to difficulty in their treatment. Natural adjuvant compounds can be co-administered to hinder the pathogenesis of resistant bacteria. Sotolon is the prevailing aromatic compound that gives fenugreek its typical smell. In the current work, the anti-virulence activities of sotolon on have been evaluated. has been treated with sotolon at sub-minimum inhibitory concentration (MIC), and production of biofilm and other virulence factors were assessed. Moreover, the anti-quorum sensing (QS) activity of sotolon was in-silico evaluated by evaluating the affinity of sotolon to bind to QS receptors, and the expression of QS genes was measured in the presence of sotolon sub-MIC. Furthermore, the sotolon in-vivo capability to protect mice against was assessed. Significantly, sotolon decreased the production of bacterial biofilm and virulence factors, the expression of QS genes, and protected mice from . Conclusively, the plant natural substance sotolon attenuated the pathogenicity of locating it as a plausible potential therapeutic agent for the treatment of its infections. Sotolon can be used in the treatment of bacterial infections as an alternative or adjuvant to antibiotics to combat their high resistance to antibiotics.
由于持续使用抗生素导致细菌耐药性的发展，使得它们的治疗变得困难。天然佐剂化合物可以联合使用以阻碍耐药细菌的发病机制。 sotolon是赋予胡芦巴典型气味的主要芳香化合物。在当前的工作中，已经评估了sotolon对[此处原文缺失相关细菌名称]的抗毒力活性。[此处原文缺失相关细菌名称]已用低于最低抑菌浓度（MIC）的sotolon处理，并评估了生物膜和其他毒力因子的产生。此外，通过评估sotolon与群体感应（QS）受体结合的亲和力，对sotolon的抗群体感应活性进行了计算机模拟评估，并在存在低于MIC的sotolon的情况下测量了QS基因的表达。此外，评估了sotolon在体内保护小鼠免受[此处原文缺失相关细菌名称]侵害的能力。值得注意的是，sotolon降低了细菌生物膜和毒力因子的产生、QS基因的表达，并保护小鼠免受[此处原文缺失相关细菌名称]的侵害。总之，植物天然物质sotolon减弱了[此处原文缺失相关细菌名称]的致病性，将其定位为治疗其感染的一种可能的潜在治疗剂。Sotolon可作为抗生素的替代品或佐剂用于治疗细菌感染，以对抗它们对抗生素的高耐药性。
