Department of Bacteriology, Academic Field of Health Science Okayama University, Okayama 700-8558, Japan.
Department of Bacteriology, Academic Field of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan.
Toxins (Basel). 2021 Jul 5;13(7):467. doi: 10.3390/toxins13070467.
The infection caused by is associated with several diseases, including gastric cancer. Several methods for the diagnosis of infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of .
感染与包括胃癌在内的多种疾病有关。有几种方法可以诊断感染，包括内窥镜检查、尿素呼气试验和粪便抗原试验，由于该试验是一种简单且高度敏感的试验，因此常被用于检测血清抗体滴度。在这种情况下，本研究旨在发现不同抗体反应性与细菌基因组组织之间的关联。接下来进行了下一代测序，以确定具有不同反应性的四种抗原菌株的基因组序列。在常见基因上进行了搜索，并使用基因组环和点图分析进行了同源性分析。两种高反应性菌株的抗原显示出很高的基因同源性，CagA 和 VacA 的 Western blot 也显示出蛋白质的高表达水平。在反应性差的抗原菌株中，发现基因组中 基因周围发生了倒位。细菌基因组的结构可能导致患者抗体的反应性差。未来，可以通过使用用于抗体滴度试验的抗原的基因突变较少的菌株来进行更准确的血清诊断。
