Yu Jie, Chen Shuai, Zeng Ziyou, Xing Shuaibing, Chen Daiwen, Yu Bing, He Jun, Huang Zhiqing, Luo Yuheng, Zheng Ping, Mao Xiangbing, Luo Junqiu, Yan Hui
Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Sichuan Tequ Agriculture and Animal Husbandry Technology Group Co., Ltd., Chengdu 610207, China.
Animals (Basel). 2021 Jul 20;11(7):2148. doi: 10.3390/ani11072148.
Low-temperature is one of the most significant risks for the animal industry. In light of this, the present study aimed to explore the effects of low-temperature on growth performance, nutrient digestibility, myofiber types and mitochondrial function in weaned piglets. A total of sixteen 21-day-old male Duroc × Landrace × Yorkshire (DLY) piglets were randomly divided into a control group (CON, 26 ± 1 °C) and a low-temperature group (LT, 15 ± 1 °C), with eight duplicate piglets in each group. The trial period lasted for 21 days. We showed that LT not only increased the ADFI ( < 0.05), as well as increasing the diarrhea incidence and diarrhea index of weaned piglets in the early stage of the experiment ( < 0.01), but it also decreased the apparent digestibility of crude protein (CP), organic matter (OM) and dry matter (DM) ( < 0.05). Meanwhile, in the LT group, the mRNA expression of ( < 0.05) in longissimus dorsi muscle (LM) and ( < 0.01) in psoas muscle (PM) were increased, while the mRNA expression of in PM was decreased ( < 0.05). In addition, LT increased the mRNA expression of mitochondrial function-related genes citrate synthase (CS) and succinate dehydrogenase-b (SDHB) in LM, as well as increased the mRNA expression of CS ( < 0.05) and carnitine palmitoyl transferase-1b (CPT-1b) ( < 0.01) in PM. Furthermore, LT increased the T-AOC activity in serum and LM ( < 0.01), as well as increased the T-SOD activity in PM ( < 0.05). Taken together, these findings showed that low-temperature could negatively affect the growth performance and nutrient digestibility, but resulted in a shift toward oxidative muscle fibers, which may occur through mitochondrial function regulation.
低温是畜牧业面临的最重要风险之一。鉴于此，本研究旨在探讨低温对断奶仔猪生长性能、养分消化率、肌纤维类型和线粒体功能的影响。选取16头21日龄的杜洛克×长白×大白（DLY）雄性仔猪，随机分为对照组（CON，26±1℃）和低温组（LT，15±1℃），每组8个重复仔猪。试验期持续21天。结果表明，低温不仅增加了平均日采食量（ADFI）（P<0.05），且在实验前期增加了断奶仔猪的腹泻发生率和腹泻指数（P<0.01），还降低了粗蛋白（CP）、有机物（OM）和干物质（DM）的表观消化率（P<0.05）。同时，在低温组中，背最长肌（LM）中[基因名称未给出]的mRNA表达增加（P<0.05），腰大肌（PM）中[基因名称未给出]的mRNA表达增加（P<0.01），而PM中[基因名称未给出]的mRNA表达降低（P<0.05）。此外，低温增加了LM中线粒体功能相关基因柠檬酸合酶（CS）和琥珀酸脱氢酶b（SDHB）的mRNA表达，以及PM中CS（P<0.05）和肉碱棕榈酰转移酶-1b（CPT-1b）（P<0.01）的mRNA表达。此外，低温增加了血清和LM中的总抗氧化能力（T-AOC）活性（P<0.01），以及PM中的超氧化物歧化酶（T-SOD）活性（P<0.05）。综上所述，这些结果表明低温会对生长性能和养分消化率产生负面影响，但会导致向氧化型肌纤维转变，这可能是通过线粒体功能调节实现的。
