Balázs Viktória Lilla, Nagy-Radványi Lilla, Filep Rita, Kerekes Erika, Kocsis Béla, Kocsis Marianna, Farkas Ágnes
Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, 7624 Pécs, Hungary.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6720 Szeged, Hungary.
Foods. 2021 Jul 14;10(7):1632. doi: 10.3390/foods10071632.
Honey is a rich source of carbohydrates, while minor compounds such as amino acids and polyphenols contribute to its health-promoting effects. Honey is one of the oldest traditional remedies applied for microbial infections, due to its antibacterial, anti-inflammatory, and antioxidant properties. The aim of this study was to investigate the antibacterial and antibiofilm effects of Hungarian black locust, linden, and sunflower honeys against the most common biofilm-forming respiratory tract pathogens spp., , and . The unifloral character of all three honey types was confirmed by melissopalynological analysis. The antibacterial activity of each honey sample against each bacterium strain was proven with agar well diffusion assay and thin layer chromatography-direct bioautography. Kinetics and mechanisms of antibacterial action were clarified with time-kill assay and membrane degradation study. The anti-biofilm activity was evidenced using crystal violet assay. In each assay, linden honey was the most effective, followed by sunflower and black locust honey. In addition, each honey sample had greater potential to suppress respiratory tract bacteria, compared to major sugar components. In conclusion, honey in general and linden honey in particular, can have a role in the treatment of respiratory tract infections caused by biofilm-forming bacteria.
蜂蜜是碳水化合物的丰富来源，而氨基酸和多酚等微量化合物则有助于其促进健康的功效。由于具有抗菌、抗炎和抗氧化特性，蜂蜜是用于治疗微生物感染的最古老的传统药物之一。本研究的目的是调查匈牙利刺槐蜜、椴树蜜和向日葵蜜对最常见的形成生物膜的呼吸道病原体、和的抗菌和抗生物膜作用。通过蜂蜜花粉分析证实了这三种蜂蜜的单一花源特性。用琼脂孔扩散法和薄层色谱-直接生物自显影法证明了每个蜂蜜样品对每种细菌菌株的抗菌活性。通过时间-杀菌试验和膜降解研究阐明了抗菌作用的动力学和机制。用结晶紫试验证明了抗生物膜活性。在每项试验中，椴树蜜最有效，其次是向日葵蜜和刺槐蜜。此外，与主要糖类成分相比，每个蜂蜜样品抑制呼吸道细菌的潜力更大。总之，一般的蜂蜜，特别是椴树蜜，在治疗由形成生物膜的细菌引起的呼吸道感染中可以发挥作用。
