Balázs Viktória L, Nagy-Radványi Lilla, Bencsik-Kerekes Erika, Koloh Regina, Szabó Dina, Kocsis Béla, Kocsis Marianna, Farkas Ágnes
Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, 7624 Pécs, Hungary.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary.
Microorganisms. 2023 Feb 17;11(2):509. doi: 10.3390/microorganisms11020509.
Honey is known as an alternative remedy for the treatment of wounds. To evaluate the potential of five Hungarian honey types against wound-associated bacteria, in vitro microbiological assays were conducted on and methicillin-resistant (MRSA). Minimum inhibitory concentration (MIC) was determined with the broth macrodilution method, and biofilm degradation capacity was tested with a crystal violet assay. To understand the underlying mechanisms, the effects of honey treatments were assessed on bacterial membrane integrity and quorum sensing (QS). The highest antibacterial activity, indicated by the lowest MIC values, as well as the highest biofilm inhibition rates and membrane disruption, was displayed by chestnut and linden honeys. The most sensitive bacterium was . Bacterial membrane degradation took place 40 min after treatment with honey solutions of at least a 40% concentration. Each honey sample exhibited anti-QS activity, which was most pronounced in the case of chestnut honey. It was concluded that the antibacterial, biofilm-inhibiting and anti-QS activities of linden and chestnut honeys were superior to those of acacia, goldenrod and milkweed honeys. In addition to the floral source, the antibacterial effect of honey is influenced by the microbial species treated. The use of honey in wound treatment can be justified by its diverse antibacterial mechanisms.
蜂蜜被认为是治疗伤口的一种替代疗法。为了评估五种匈牙利蜂蜜对伤口相关细菌的潜在作用，对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）进行了体外微生物学测定。采用肉汤稀释法测定最低抑菌浓度（MIC），并通过结晶紫试验检测生物膜降解能力。为了了解其潜在机制，评估了蜂蜜处理对细菌膜完整性和群体感应（QS）的影响。栗树蜜和椴树蜜表现出最高的抗菌活性，表现为最低的MIC值、最高的生物膜抑制率和膜破坏。最敏感的细菌是金黄色葡萄球菌。用至少40%浓度的蜂蜜溶液处理40分钟后，细菌膜发生降解。每个蜂蜜样品都表现出抗群体感应活性，在栗树蜜中最为明显。得出的结论是，椴树蜜和栗树蜜的抗菌、生物膜抑制和抗群体感应活性优于刺槐蜜、一枝黄花蜜和马利筋蜜。除了花蜜来源外，蜂蜜的抗菌效果还受所处理微生物种类的影响。蜂蜜在伤口治疗中的应用因其多样的抗菌机制而合理。
