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SmSPL6 诱导酚酸生物合成并影响. 的根发育。

SmSPL6 Induces Phenolic Acid Biosynthesis and Affects Root Development in .

机构信息

Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi'an 710062, China.

出版信息

Int J Mol Sci. 2021 Jul 23;22(15):7895. doi: 10.3390/ijms22157895.

Abstract

is a renowned model medicinal plant species for which 15 family genes have been identified; however, the specific functions of have not been well characterized as of yet. For this study, the expression patterns of were determined through its responses to treatments of exogenous hormones, including indole acetic acid (IAA), gibberellic acid (GA), methyl jasmonic acid (MeJA), and abscisic acid (ABA). To characterize its functionality, we obtained ovexpressed transgenic plants and found that overexpressed promoted the accumulation of phenolic acids and repressed the biosynthesis of anthocyanin. Meanwhile, the root lengths of the -overexpressed lines were significantly longer than the control; however, both the fresh weights and lateral root numbers decreased. Further investigations indicated that SPL6 regulated the biosynthesis of phenolic acid by directly binding to the promoter regions of the enzyme genes and and activated their expression. We concluded that SPL6 regulates not only the biosynthesis of phenolic acids, but also the development of roots in .

摘要

是一种著名的药用植物模式种,已经鉴定出 15 个家族基因;然而,其具体功能尚未得到很好的表征。在这项研究中,通过其对外源激素(包括吲哚乙酸(IAA)、赤霉素(GA)、茉莉酸甲酯(MeJA)和脱落酸(ABA))的反应来确定 的表达模式。为了表征其功能,我们获得了过表达的转基因 植物,发现过表达 促进了酚酸的积累,并抑制了花青素的生物合成。同时, -过表达系的根长明显长于对照;然而,鲜重和侧根数都减少了。进一步的研究表明,SPL6 通过直接结合酶基因 和 的启动子区域来调节酚酸的生物合成,并激活它们的表达。我们得出结论,SPL6 不仅调节酚酸的生物合成,还调节 的根发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cb2/8348295/d05a2f2c66fe/ijms-22-07895-g001.jpg

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