Laboratory of Medicinal Plant Biotechnology, College of pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, PR China; Institute of Plant Biotechnology, School of Life Sciences, Shanghai Normal University, Shanghai 200234, PR China.
Laboratory of Medicinal Plant Biotechnology, College of pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, PR China.
Food Chem. 2020 Nov 30;331:127365. doi: 10.1016/j.foodchem.2020.127365. Epub 2020 Jun 17.
Phenolic acids from Salvia miltiorrhiza have been widely used in nutritious, health-promoting products with an increasing demand. In the current study, two biosynthetic genes RAS (rosmarinic acid synthase) and CYP98A14 (a cytochrome P450-dependent monooxygenase) were successfully introduced into S. miltiorrhiza hairy roots. Overexpression of RAS and CYP98A14 resulted in higher content of phenolic acids (up to over 3-fold) in transgenic lines compared to the control. Meanwhile, DPPH results revealed that engineered S. miltiorrhiza hairy roots had stronger antioxidant activities than the control. In addition, phenolic acid crude extracts of the engineered hairy root lines overexpressing RAS or CYP98A14 showed improved antibacterial activities compared to the control lines. Our work exhibits a useful strategy for enhancement of phenolic acid production and bioactivities of S. miltiorrhiza hairy roots by genetic manipulation of RAS and CYP98A14, and also provides a new resource material to obtain active phenolic acids for food and healthy products.
丹参中的酚酸类物质被广泛应用于营养保健品中，需求量日益增加。在本研究中，成功将两个生物合成基因 RAS（迷迭香酸合酶）和 CYP98A14（细胞色素 P450 依赖的单加氧酶）导入丹参毛状根。与对照相比，RAS 和 CYP98A14 的过表达导致转基因系中酚酸含量增加（高达 3 倍以上）。同时，DPPH 结果表明，工程化的丹参毛状根具有比对照更强的抗氧化活性。此外，与对照系相比，过表达 RAS 或 CYP98A14 的工程化毛状根系的酚酸粗提物表现出改善的抗菌活性。我们的工作通过对 RAS 和 CYP98A14 的遗传操作展示了一种提高丹参毛状根中酚酸产量和生物活性的有效策略，并为获得用于食品和健康产品的活性酚酸提供了新的资源材料。
