Refractories, Ceramics and Building materials Department, National Research Centre, 33El Bohouth St. (former EL Tahrir St.), Dokki, P.O.12622, Giza, Egypt.
Refractories, Ceramics and Building materials Department, National Research Centre, 33El Bohouth St. (former EL Tahrir St.), Dokki, P.O.12622, Giza, Egypt.
Biomater Adv. 2022 Jul;138:212965. doi: 10.1016/j.bioadv.2022.212965. Epub 2022 May 30.
The hydration state of bioactive glass materials and its relationship with their biocompatibility have been receiving attention. In this research, silver-containing bioactive glasses (BGAgs) (Ag contents of 0.25, 0.5, and 1.0% in the glass system) were developed using the sol-gel method. Their physicochemical properties, size, morphology, and surface area were characterized by conducting X-rays diffraction (XRD), Fourier transform infrared (FTIR), Transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) surface area analyses. The surface charges of the developed BGAgs were evaluated using the Nano Zetasizer. Moreover, the antibacterial activities and intermediate water (IW) contents of hydrated BGAgs were determined. Finally, BGAgs disks were tested against osteosarcoma (MG63) cell line to evaluate their death modes. The physicochemical characteristics of the BGAgs revealed no modifications after Ag doping. In comparison, relative changes were recorded in the particle size (20-33 to 16-29 nm), surface area (4.3 to 3.7 m/g), and particle charge (-24 to -14.6 mV). Doping the current glass system with silver produced impressive amounts of IW, consistent with recorded proliferation rates of the cells when treated with BGAgs. The determined hydration states correlated with other findings in this research might be helpful in predicting and assessing the biological behaviors of BGAgs.
生物活性玻璃材料的水合状态及其与生物相容性的关系一直受到关注。本研究采用溶胶-凝胶法制备了含银生物活性玻璃（BGAgs）（玻璃体系中 Ag 的含量分别为 0.25%、0.5%和 1.0%）。通过 X 射线衍射（XRD）、傅里叶变换红外（FTIR）、透射电子显微镜（TEM）和 Brunauer-Emmett-Teller（BET）表面积分析对其物理化学性质、尺寸、形态和表面积进行了表征。使用纳米粒度仪评估了开发的 BGAgs 的表面电荷。此外，还测定了水合 BGAgs 的抗菌活性和中间水（IW）含量。最后，用 BGAgs 圆盘测试了骨肉瘤（MG63）细胞系，以评估其死亡模式。BGAgs 的物理化学特性在 Ag 掺杂后没有发生变化。相比之下，粒径（20-33 至 16-29nm）、表面积（4.3 至 3.7m/g）和颗粒电荷（-24 至-14.6mV）发生了相对变化。在当前的玻璃体系中掺杂银产生了大量的 IW，与用 BGAgs 处理细胞时记录的增殖率一致。所确定的水合状态与本研究中的其他发现相关，这可能有助于预测和评估 BGAgs 的生物学行为。
