Muro Antonio, Belhassen-García Moncef, Muñoz Bellido Juan Luís, Lorenzo Juanes Helena, Vicente Belén, Pendones Josué, Adserias José, Sánchez Hernández Gonzalo, Rodríguez Rosa Miguel, Vicente Villardón José Luis, Burguillo Javier, López Andaluz Javier, Martín Oterino Jose Angel, García Criado Francisco Javier, Barbero Fausto, Morales Ana Isabel, Galindo Villardón Purificación, González Sarmiento Rogelio
Infectious and Tropical Diseases Group (e-INTRO), Institute of Biomedical Research of Salamanca-Research Center for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37008 Salamanca, Spain.
Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, University Hospital of Salamanca, 37007 Salamanca, Spain.
J Clin Med. 2021 Jul 21;10(15):3214. doi: 10.3390/jcm10153214.
Systematic screening for antibodies against SARS-CoV-2 is a crucial tool for surveillance of the COVID-19 pandemic. The University of Salamanca (USAL) in Spain designed a project called "DIANCUSAL" (Diagnosis of New Coronavirus, COVID-19, in University of Salamanca) to measure antibodies against SARS-CoV-2 among its ~34,000 students and academic staff, as the influence of the university community in the spread of the SARS-CoV-2 pandemic in the city of Salamanca and neighboring towns hosting USAL campuses could be substantial.
The aim of this study was to estimate the prevalence of SARS-CoV-2 antibodies among USAL students, professors and staff and to evaluate the demographic, academic, clinical and lifestyle and behavioral factors related to seropositivity.
The DIANCUSAL study is an ongoing university population-based cross-sectional study, with the work described herein conducted from July-October 2020. All USAL students, professors and staff were invited to complete an anonymized questionnaire. Seroprevalence of anti-SARS-CoV-2 antibodies was detected and quantified by using chemiluminescent assays for IgG and IgM.
A total of 8197 (24.71%) participants were included. The mean age was 31.4 (14.5 SD) years, and 66.0% of the participants were female. The seroprevalence was 8.25% overall and was highest for students from the education campus (12.5%) and professors from the biomedical campus (12.6%), with significant differences among faculties ( = 0.006). Based on the questionnaire, loss of smell and fever were the symptoms most strongly associated with seropositivity, and 22.6% of seropositive participants were asymptomatic. Social distancing was the most effective hygiene measure ( = 0.0007). There were significant differences in seroprevalence between participants with and without household exposure to SARS-CoV-2 ( = 0.0000), but not between students who lived in private homes and those who lived in dormitories. IgG antibodies decreased over time in the participants with confirmed self-reported COVID-19 diagnoses.
The analysis revealed an overall 8.25% seroprevalence at the end of October 2020, with a higher seroprevalence in students than in staff. Thus, there is no need for tailored measures for the USAL community as the official average seroprevalence in the area was similar (7.8% at 22 June and 12.4 at 15 November of 2020). Instead, USAL members should comply with public health measures.
对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）抗体进行系统筛查是监测新冠疫情的一项关键工具。西班牙萨拉曼卡大学（USAL）设计了一个名为“DIANCUSAL”（萨拉曼卡大学新型冠状病毒2019冠状病毒病诊断）的项目，以检测其约34000名学生和学术人员体内的SARS-CoV-2抗体，因为大学社区对SARS-CoV-2疫情在萨拉曼卡市及设有USAL校区的周边城镇传播的影响可能很大。
本研究旨在估计USAL学生、教授和工作人员中SARS-CoV-2抗体的流行率，并评估与血清阳性相关的人口统计学、学术、临床、生活方式和行为因素。
DIANCUSAL研究是一项正在进行的基于大学人群的横断面研究，本文所述工作于2020年7月至10月开展。邀请所有USAL学生、教授和工作人员填写一份匿名问卷。采用化学发光法检测和定量抗SARS-CoV-2抗体的血清阳性率，检测IgG和IgM。
共纳入8197名（24.71%）参与者。平均年龄为31.4（标准差14.5）岁，66.0%的参与者为女性。总体血清阳性率为8.25%，教育校区的学生（12.5%）和生物医学校区的教授（12.6%）血清阳性率最高，各学院之间存在显著差异（P = 0.006）。根据问卷，嗅觉丧失和发热是与血清阳性最密切相关的症状，22.6%的血清阳性参与者无症状。保持社交距离是最有效的卫生措施（P = 0.0007）。有SARS-CoV-2家庭接触史和无家庭接触史的参与者之间血清阳性率存在显著差异（P = 0.0000），但居住在私人住宅的学生和居住在宿舍的学生之间无差异。在确诊为自我报告的新冠病例的参与者中，IgG抗体随时间下降。
分析显示，截至2020年10月底，总体血清阳性率为8.25%，学生的血清阳性率高于工作人员。因此，由于该地区官方平均血清阳性率相似（2020年6月22日为7.8%，11月15日为12.4%），无需为USAL社区采取针对性措施。相反，USAL成员应遵守公共卫生措施。
