Rogos R, Appelt G, Möller T, Wegner D
Forschungsabteilung Gastroenterologie, Bereichs Medizin, Karl-Marx-Universität Leipzig.
Dtsch Z Verdau Stoffwechselkr. 1987;47(5):242-51.
A modified secretin-pancreozymin test was performed for the quantitative determination of both the pancreatic exocrine function and the biliary secretion of total bile acids followed by exogenous (secretin and pancreozymin) and endogenous (L-phenylalanine) stimulation of the pancreatic and biliary secretion. The outputs of lipase, alpha-amylase, trypsin, bicarbonate and total bile acids were estimated and the ratio of glycine to taurine conjugated bile acids was measured by thin layer chromatography. Polyethylene glycol 4,000 was used as a nonabsorbable marker for the correction of aspirated volumes. 40 patients were studied: 10 control subjects; 10 control subjects with previous cholecystectomy; 10 patients with chronic pancreatitis and 10 cholecystectomized patients with chronic pancreatitis. In cholecystectomized probands, not only the total bile acid output but also the output of bicarbonate, trypsin and lipase were diminished compared to the control subjects with a intact gallbladder. In noncholecystectomized and cholecystectomized patients with chronic pancreatitis both, the pancreatic secretion and the biliary bile acid secretion were significantly decreased. The ratio of glycine/taurine conjugated bile acids was found to be significantly higher in these patients compared to the controls.
进行了改良的促胰液素-胰酶泌素试验，以定量测定胰腺外分泌功能和胆汁中总胆汁酸的分泌，随后通过外源性（促胰液素和胰酶泌素）和内源性（L-苯丙氨酸）刺激胰腺和胆汁分泌。估计脂肪酶、α-淀粉酶、胰蛋白酶、碳酸氢盐和总胆汁酸的分泌量，并通过薄层色谱法测量甘氨酸与牛磺酸结合胆汁酸的比例。聚乙二醇4000用作不可吸收标记物，用于校正吸出体积。研究了40名患者：10名对照受试者；10名曾行胆囊切除术的对照受试者；10名慢性胰腺炎患者和10名行胆囊切除术的慢性胰腺炎患者。与胆囊完整的对照受试者相比，行胆囊切除术的先证者不仅总胆汁酸分泌量减少，而且碳酸氢盐、胰蛋白酶和脂肪酶的分泌量也减少。在未行胆囊切除术和行胆囊切除术的慢性胰腺炎患者中，胰腺分泌和胆汁胆汁酸分泌均显著减少。与对照组相比，这些患者中甘氨酸/牛磺酸结合胆汁酸的比例显著更高。
