Pittius C W, Ellendorff F, Höllt V, Parvizi N
Institut für Neuropharmakologie, Max-Planck-Institut für Psychiatrie, Martinsried, Federal Republic of Germany.
Exp Brain Res. 1987;69(1):208-12. doi: 10.1007/BF00247043.
Using solution hybridization analysis and Northern blotting with complementary RNA probes labelled to high specific activity, levels of proenkephalin A and B mRNA were analyzed throughout prenatal development in the hippocampus and striatum of fetal pigs. A differential time course for the appearance of these opioid precursor mRNAs was observed: in hippocampus, both mRNAs increased linearly throughout development with proenkephalin B mRNA increasing faster than proenkephalin A mRNA. In striatum, both mRNAs behaved similarly, increasing to a maximum level around mid-gestation and declining thereafter. The differences might be attributed to differential localization of the two precursor systems in the tissues and might be of functional relevance.
利用溶液杂交分析和用高比活性标记的互补RNA探针进行Northern印迹法，分析了胎猪海马和纹状体在整个产前发育过程中脑啡肽原A和B mRNA的水平。观察到这些阿片样物质前体mRNA出现的时间进程不同：在海马中，两种mRNA在整个发育过程中呈线性增加，脑啡肽原B mRNA的增加速度比脑啡肽原A mRNA快。在纹状体中，两种mRNA表现相似，在妊娠中期左右增加到最高水平，然后下降。这些差异可能归因于两种前体系统在组织中的不同定位，并且可能具有功能相关性。
