Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Diseases, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.
Carbohydr Polym. 2021 Oct 15;270:118348. doi: 10.1016/j.carbpol.2021.118348. Epub 2021 Jun 17.
Rapid and effective hemorrhage control is essential to reduce mortality following traumatic injuries. Herein we developed an organic solvent-free process to prepare carboxymethyl chitin microsphere (CMCHm) in an aqueous two-phase system through heating and freeze-drying. To further enhance the hemostatic performance of CMCHm, we loaded calcium ions and in-situ polymerized dopamine to get modified hemostatic microspheres CMCHm-Ca and CMCHm-PDA, respectively. The size of these microspheres was mainly distributed between 50 μm and 150 μm, and the porous microstructure was observed by SEM. The data of in vitro degradation, cell cytotoxicity, and hemolysis test indicated good biocompatibility of these microspheres. Importantly, CMCHm-Ca and CMCHm-PDA displayed better hemostatic performance compared with CMCHm and the positive controls Yunnan baiyao® and Quickclean®. Especially, the bleeding time was reduced to 59 s (CMCHm-Ca) and 45 s (CMCHm-PDA) in the femoral artery/vein cut model, respectively. All these demonstrate CMCHm-Ca and CMCHm-PDA hold great potential for rapid hemostasis.
迅速有效地控制出血对于降低创伤后死亡率至关重要。在此，我们开发了一种无有机溶剂的方法，在水相双体系中通过加热和冻干制备羧甲基壳聚糖微球（CMCHm）。为了进一步提高 CMCHm 的止血性能，我们分别负载钙离子和原位聚合多巴胺，得到改性止血微球 CMCHm-Ca 和 CMCHm-PDA。这些微球的粒径主要分布在 50μm 和 150μm 之间，通过 SEM 观察到多孔的微结构。体外降解、细胞毒性和溶血试验的数据表明这些微球具有良好的生物相容性。重要的是，与 CMCHm 以及阳性对照云南白药®和 Quickclean®相比，CMCHm-Ca 和 CMCHm-PDA 表现出更好的止血性能。特别是在股动静脉切开模型中，CMCHm-Ca 和 CMCHm-PDA 的出血时间分别缩短至 59s 和 45s。所有这些都表明 CMCHm-Ca 和 CMCHm-PDA 在快速止血方面具有巨大的潜力。
