Zhao Jiebing, Tian Hao, Shang Fusheng, Lv Tao, Chen Dagui, Feng Jianjun
Department of Orthopedics, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China.
Institute of Translational Medicine, Shanghai University, Shanghai 200444, China.
J Funct Biomater. 2022 Jul 10;13(3):91. doi: 10.3390/jfb13030091.
The purpose of this study is to fabricate different anti-cancer drug-eluted chitosan microspheres for combination therapy of osteosarcoma. In this study, electrospray in combination with ground liquid nitrogen was utilized to manufacture the microspheres. The size of obtained chitosan microspheres was uniform, and the average diameter was 532 μm. The model drug release rate and biodegradation rate of chitosan microspheres could be controlled by the glutaraldehyde vapor crosslinking time. Then the 5-fluorouracil (5-FU), paclitaxel (PTX), and Cis-dichlorodiammine-platinum (CDDP) eluted chitosan microspheres were prepared, and two osteosarcoma cell lines, namely, HOS and MG-63, were selected as cell models for in vitro demonstration. We found the 5-FU microspheres, PTX microspheres, and CDDP microspheres could significantly inhibit the growth and migration of both HOS and MG-63 cells. The apoptosis of both cells treated with 5-FU microspheres, PTX microspheres, and CDDP microspheres was significantly increased compared to the counterparts of control and blank groups. The anti-cancer drug-eluted chitosan microspheres show great potential for the treatment of osteosarcoma.
本研究的目的是制备不同的抗癌药物洗脱壳聚糖微球，用于骨肉瘤的联合治疗。在本研究中，采用电喷雾结合液氮研磨来制备微球。所获得的壳聚糖微球尺寸均匀，平均直径为532μm。壳聚糖微球的模型药物释放率和生物降解率可通过戊二醛蒸汽交联时间来控制。然后制备了5-氟尿嘧啶（5-FU）、紫杉醇（PTX）和顺铂（CDDP）洗脱的壳聚糖微球，并选择两种骨肉瘤细胞系，即HOS和MG-63，作为体外验证的细胞模型。我们发现5-FU微球、PTX微球和CDDP微球均可显著抑制HOS和MG-63细胞的生长和迁移。与对照组和空白组相比，用5-FU微球、PTX微球和CDDP微球处理的两种细胞的凋亡均显著增加。抗癌药物洗脱壳聚糖微球在骨肉瘤治疗中显示出巨大潜力。
