Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, Zhejiang, China.
J Nanobiotechnology. 2023 Jun 6;21(1):178. doi: 10.1186/s12951-023-01936-w.
Microneedle patches have been extensively employed for wound healing, while the lack of rapid hemostasis efficiency and multiple tissue-repair properties restrict their values in hemorrhagic wound applications. Herein, we propose a Yunnan Baiyao-loaded multifunctional microneedle patch, namely (BY + EGF)@MN, with deep tissue penetration, hemostasis efficiency and regenerative properties for hemorrhagic wound healing. The (BY + EGF)@MNs are designed with a BY-loaded Bletilla striata polysaccharide (BSP) base for rapid hemostasis and epidermal growth factor (EGF)-loaded GelMA tips for subsequent wound healing. As the BSP base can be fastly dissolved and completely release BY in 6 min to promote platelet adhesion and activate coagulation system, while the EGF can achieve a controlled and sustained release behavior in 7 days with the gradual degradation of the GelMA tips, the (BY + EGF)@MNs exhibit strong pro-coagulability and satisfactory hemostatic effect in a rat hepatic hemorrhage wound model. Based on the multifunctional characteristics, we have verified that when applied in rat cutaneous wounds, the proposed MNs can accelerate the wound healing process by enhancing neovascularization, fibroblast density, and collagen deposition. Thus, we believe that such (BY + EGF)@MNs are promising candidates for rapid hemostasis and diverse wound healing applications.
微针贴片在伤口愈合方面得到了广泛应用，但由于缺乏快速止血效率和多种组织修复特性，限制了其在出血性伤口应用中的价值。在此，我们提出了一种载云南白药的多功能微针贴片，即 (BY+EGF)@MN，它具有深层组织穿透性、止血效率和再生特性，可用于出血性伤口愈合。(BY+EGF)@MNs 设计有载云南白药的白芨多糖 (BSP) 基底，用于快速止血，以及载表皮生长因子 (EGF) 的 GelMA 尖端，用于后续伤口愈合。由于 BSP 基底可在 6 分钟内迅速溶解并完全释放 BY，从而促进血小板黏附并激活凝血系统，而 EGF 可通过 GelMA 尖端的逐渐降解实现 7 天的控释和持续释放行为，(BY+EGF)@MNs 在大鼠肝出血伤口模型中表现出较强的促凝性和令人满意的止血效果。基于多功能特性，我们已经验证，当应用于大鼠皮肤伤口时，所提出的 MNs 可以通过促进血管生成、成纤维细胞密度和胶原蛋白沉积来加速伤口愈合过程。因此，我们相信这种 (BY+EGF)@MN 是快速止血和多种伤口愈合应用的有前途的候选者。
