Chen Dongbin, Li Junhua, Jiao Fuchao, Wang Qianqian, Li Jun, Pei Yuhe, Zhao Meiai, Song Xiyun, Guo Xinmei
College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, China.
Front Plant Sci. 2021 Jul 23;12:593001. doi: 10.3389/fpls.2021.593001. eCollection 2021.
Aminoacylase-1 is a zinc-binding enzyme that is important in urea cycling, ammonia scavenging, and oxidative stress responses in animals. Aminoacylase-1 () has been reported to play a role in resistance to pathogen infection in the model plant . However, little is known about its function in plant growth and abiotic stress responses. In this study, we cloned and analyzed expression patterns of in under different conditions. We also functionally characterized in . We found that is expressed specifically in mature shoots compared with other tissues. is repressed by salt, drought, jasmonic acid, and salicylic acid, but is induced by abscisic acid and ethylene, indicating a potential role in stress responses and plant growth. The overexpression of in promoted growth rate by promoting growth-related genes, such as and . At the same time, the overexpression of in reduced tolerance to drought and salt stress. With drought and salt stress, the activity of protective enzymes, such as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) from micrococcus lysodeikticus was lower; while the content of malondialdehyde (MDA) and relative electrolytic leakage was higher in overexpression lines than that in wild-type lines. The results indicate that plays an important role in the balance of plant growth and defense and can be used to assist plant breeding under abiotic stress conditions.
氨基酰化酶-1是一种锌结合酶，在动物的尿素循环、氨清除和氧化应激反应中起重要作用。据报道，氨基酰化酶-1（）在模式植物对病原体感染的抗性中发挥作用。然而，关于其在植物生长和非生物胁迫反应中的功能知之甚少。在本研究中，我们克隆并分析了在不同条件下的表达模式。我们还对中的功能进行了表征。我们发现与其他组织相比，在成熟芽中特异性表达。受盐、干旱、茉莉酸和水杨酸抑制，但受脱落酸和乙烯诱导，表明其在胁迫反应和植物生长中具有潜在作用。在中过表达促进了生长相关基因如和的表达，从而提高了生长速率。同时，在中过表达降低了对干旱和盐胁迫的耐受性。在干旱和盐胁迫下，来自溶壁微球菌的过氧化物酶（POD）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等保护酶的活性较低；而过表达系中丙二醛（MDA）含量和相对电导率高于野生型系。结果表明，在植物生长与防御的平衡中起重要作用，可用于非生物胁迫条件下的植物育种辅助。
