Han Yaguang, Li Haobo, Zhou Rong, Wu Jun, Liu Ziye, Wang Huan, Shao Jiahua, Chen Yi, Zhu Jun, Fu Qiwei, Qian Qirong, Zhou Yiqin
Department of Orthopedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai 200003, China.
Department of Orthopedics, 72th Hospital of PLA, Huzhou, 313000 Zhejiang Province, China.
Stem Cells Int. 2021 Jul 27;2021:9966966. doi: 10.1155/2021/9966966. eCollection 2021.
Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic method in regenerative medicine. Our previous research adopted a simple nonenzymatic strategy for the preparation of a new type of ready-to-use infrapatellar fat pad (IPFP) cell concentrates. The aim of this study was to compare the therapeutic efficacy of intra-articular (IA) injection of autologous IPFP cell concentrates and allogeneic IPFP-MSCs obtained from these concentrates in a rabbit articular cartilage defect model. IPFP-MSCs sprouting from the IPFP cell concentrates were characterized via flow cytometry as well as based on their potential for differentiation into adipocytes, osteoblasts, and chondrocytes. In the rabbit model, cartilage defects were created on the trochlear groove, followed by treatment with IPFP cell concentrates, IPFP-MSCs, or normal saline IA injection. Distal femur samples were evaluated at 6 and 12 weeks posttreatment via macroscopic observation and histological assessment based on the International Cartilage Repair Society (ICRS) macroscopic scoring system as well as the ICRS visual histological assessment scale. The macroscopic score and histological score were significantly higher in the IPFP-MSC group compared to the IPFP cell concentrate group at 12 weeks. Further, both treatment groups had higher scores compared to the normal saline group. In comparison to the latter, the groups treated with IPFP-MSCs and IPFP cell concentrates showed considerably better cartilage regeneration. Overall, IPFP-MSCs represent an effective therapeutic strategy for stimulating articular cartilage regeneration. Further, due to the simple, cost-effective, nonenzymatic, and safe preparation process, IPFP cell concentrates may represent an effective alternative to stem cell-based therapy in the clinic.
间充质干细胞（MSCs）已成为再生医学中一种有前景的治疗方法。我们之前的研究采用了一种简单的非酶策略来制备新型即用型髌下脂肪垫（IPFP）细胞浓缩物。本研究的目的是在兔关节软骨缺损模型中比较关节内（IA）注射自体IPFP细胞浓缩物和从这些浓缩物中获得的异体IPFP-MSCs的治疗效果。通过流式细胞术以及基于其向脂肪细胞、成骨细胞和软骨细胞分化的潜力，对从IPFP细胞浓缩物中萌发的IPFP-MSCs进行了表征。在兔模型中，在滑车沟制造软骨缺损，然后用IPFP细胞浓缩物、IPFP-MSCs或生理盐水IA注射进行治疗。在治疗后6周和12周，通过基于国际软骨修复协会（ICRS）宏观评分系统以及ICRS视觉组织学评估量表的宏观观察和组织学评估，对股骨远端样本进行评估。在12周时，IPFP-MSC组的宏观评分和组织学评分显著高于IPFP细胞浓缩物组。此外，两个治疗组的评分均高于生理盐水组。与后者相比，用IPFP-MSCs和IPFP细胞浓缩物治疗的组显示出明显更好的软骨再生。总体而言，IPFP-MSCs是刺激关节软骨再生的一种有效治疗策略。此外，由于制备过程简单、成本效益高、非酶且安全，IPFP细胞浓缩物可能是临床上基于干细胞治疗的一种有效替代方法。
