Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, P. R. China.
Anal Chem. 2021 Aug 17;93(32):11298-11304. doi: 10.1021/acs.analchem.1c02489. Epub 2021 Aug 8.
Small extracellular vesicles (sEVs), often referred to as exosomes, are potential biomarkers for noninvasive cancer diagnosis. However, because of their phenotype heterogeneity, precise detection of tumor-derived sEVs is a great challenge. Herein, a dual-aptamer-assisted AND logic gate was fabricated for sensitive electrochemical detection of tumor-derived sEVs based on a cyclic enzymatic signal amplification strategy. Four different tumor-derived sEVs were used to verify the feasibility of the AND logic gate, and CCRF-CEM sEVs were successfully detected by this assay. The electrochemical assay shows a good linear response from 4 × 10 to 8 × 10 particles/μL, with a detection limit of 920 particles/μL, for CCRF-CEM sEVs, indicating potential application in accurate cancer diagnostics.
小细胞外囊泡（sEVs），通常被称为外泌体，是用于非侵入性癌症诊断的有潜力的生物标志物。然而，由于其表型异质性，精确检测肿瘤来源的 sEVs 是一个巨大的挑战。在此，构建了一种基于循环酶促信号放大策略的双适体辅助的与门，用于灵敏的电化学检测肿瘤来源的 sEVs。使用四种不同的肿瘤来源的 sEVs 来验证与门的可行性，并通过该测定法成功检测到 CCRF-CEM sEVs。电化学测定法对 CCRF-CEM sEVs 的线性响应范围从 4×10 到 8×10 个颗粒/μL，检测限为 920 个颗粒/μL，表明其在准确癌症诊断中有潜在应用。
