Institute of Metabolism and Systems Research, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
School of Science and Technology, Nottingham Trent University, Nottingham, UK.
J Endocrinol. 2021 Sep 9;251(1):111-123. doi: 10.1530/JOE-21-0123.
Supplementation with precursors of NAD has been shown to prevent and reverse insulin resistance, mitochondrial dysfunction, and liver damage in mouse models of diet-induced obesity. We asked whether the beneficial effects of supplementation with the NAD precursor nicotinamide riboside (NR) are dependent on mouse strain. We compared the effects of NR supplementation on whole-body energy metabolism and mitochondrial function in mildly obese C57BL/6N and C57BL/6J mice, two commonly used strains to investigate metabolism. Male C57BL/6N and C57BL/6J mice were fed a high-fat diet (HFD) or standard chow with or without NR supplementation for 8 weeks. Body and organ weights, glucose tolerance, and metabolic parameters as well as mitochondrial O2 flux in liver and muscle fibers were assessed. We found that NR supplementation had no influence on body or organ weight, glucose metabolism or hepatic lipid accumulation, energy expenditure, or metabolic flexibility but increased mitochondrial respiration in soleus muscle in both mouse strains. Strain-dependent differences were detected for body and fat depot weight, fasting blood glucose, hepatic lipid accumulation, and energy expenditure. We conclude that, in mild obesity, NR supplementation does not alter metabolic phenotype in two commonly used laboratory mouse strains.
补充 NAD 前体已被证明可以预防和逆转饮食诱导肥胖的小鼠模型中的胰岛素抵抗、线粒体功能障碍和肝损伤。我们想知道补充 NAD 前体烟酰胺核糖（NR）的有益效果是否依赖于小鼠品系。我们比较了 NR 补充对两种常用代谢研究品系——轻度肥胖的 C57BL/6N 和 C57BL/6J 小鼠的全身能量代谢和线粒体功能的影响。雄性 C57BL/6N 和 C57BL/6J 小鼠喂食高脂肪饮食（HFD）或标准饲料，或同时补充 NR，持续 8 周。评估体重和器官重量、葡萄糖耐量和代谢参数以及肝脏和肌纤维中的线粒体 O2 流量。我们发现，NR 补充对体重或器官重量、葡萄糖代谢或肝脂质积累、能量消耗或代谢灵活性没有影响，但增加了两种小鼠品系的比目鱼肌中的线粒体呼吸。两种小鼠品系的体重和脂肪储存量、空腹血糖、肝脂质积累和能量消耗存在品系依赖性差异。我们得出结论，在轻度肥胖中，NR 补充不会改变两种常用实验室小鼠品系的代谢表型。
