Lee Moon Hyun, Lee Dae Ryeong, Chon Joon Woo, Chung Dong June
Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon 16419, Korea.
Polymers (Basel). 2021 Jul 22;13(15):2402. doi: 10.3390/polym13152402.
In this study, we manufactured biocompatible hemostatic crosslinked chitosan (CS) patches and analyzed their physicochemical and biological properties for femoral arterial puncture applications. CS is a representative hemostatic material but has some drawbacks, such as swelling, shrinkage, and brittleness. Thus, it was crosslinked via a 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/-hydroxysuccinimide (NHS) coupling reaction and a nucleophilic addition reaction with citric acid (CA), glutaraldehyde (GTA), and genipin (GP) to remedy its shortcomings. The CSCA (crosslinked CS with CA/EDC), CSGTA (crosslinked CS with GTA), and CSG (crosslinked CS with GP) films showed low swelling degrees and good mechanical properties (excluding CSCA) compared with those of neat CS films. Additionally, every crosslinked CS film coated with thrombin (TB-CS) showed enhanced hemostatic ability in the whole blood clotting and activated partial thromboplastin time tests. Furthermore, the CSCA, CSGTA, and CSGP were nontoxic in an in vitro cell cytotoxicity test (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay) using L-929 mouse fibroblasts cells.
在本研究中，我们制备了具有生物相容性的止血交联壳聚糖（CS）贴片，并分析了其用于股动脉穿刺的物理化学和生物学特性。CS是一种典型的止血材料，但存在一些缺点，如肿胀、收缩和脆性。因此，通过1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）/N-羟基琥珀酰亚胺（NHS）偶联反应以及与柠檬酸（CA）、戊二醛（GTA）和京尼平（GP）的亲核加成反应对其进行交联，以弥补其缺点。与纯CS膜相比，CSCA（CS与CA交联）、CSGTA（CS与GTA交联）和CSG（CS与GP交联）膜表现出较低的溶胀度和良好的机械性能（不包括CSCA）。此外，在全血凝血和活化部分凝血活酶时间测试中，每一种涂有凝血酶的交联CS膜（TB-CS）均表现出增强的止血能力。此外，在使用L-929小鼠成纤维细胞的体外细胞毒性试验（3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐法）中，CSCA、CSGTA和CSGP均无毒。
