Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Central Research Laboratory, Seikagaku Corporation, Higashiyamato-shi, Japan.
FEBS Lett. 2021 Sep;595(18):2341-2349. doi: 10.1002/1873-3468.14173. Epub 2021 Aug 17.
Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS-CoV-2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high-sensitivity screening of the GAG-binding specificity of proteins and applied it for the analysis of SARS-CoV-2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration-dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS-CoV-2 infection.
硫酸乙酰肝素 (HS) 是一种带有磺酸基团的糖胺聚糖 (GAG)，据报道它是一种必要的宿主附着因子，可促进 SARS-CoV-2 感染。在这项研究中，我们开发了基于荧光检测的 GAG 微阵列，用于高灵敏度筛选蛋白质与 GAG 的结合特异性，并将其应用于 SARS-CoV-2 刺突 (S) 蛋白的分析。在 20 种不同的 GAG 中，S 蛋白不仅与肝素 (HEP)/HS 结合，而且还以浓度依赖的方式与硫酸软骨素 E (CSE) 结合。然后，我们分析了 S 蛋白每个亚基的特异性。虽然 S1 亚基表现出与 HEP 的特异性结合，但 S2 亚基也与 CSE 和 HEP/HS 结合。CSE 可能在 SARS-CoV-2 感染中作为 HS 的替代附着因子。
