Physiologie moléculaire et adaptation UMR 7221 CNRS and Muséum National d'Histoire Naturelle, Paris, France.
Mécanismes adaptatifs et évolution UMR 7179 CNRS and Muséum National d'Histoire Naturelle, Paris, France.
Open Biol. 2021 Aug;11(8):210065. doi: 10.1098/rsob.210065. Epub 2021 Aug 11.
Urp1 and Urp2 are two neuropeptides of the urotensin II family identified in teleost fish and mainly expressed in cerebrospinal fluid (CSF)-contacting neurons. It has been recently proposed that Urp1 and Urp2 are required for correct axis formation and maintenance. Their action is thought to be mediated by the receptor Uts2r3, which is specifically expressed in dorsal somites. In support of this view, it has been demonstrated that the loss of results in severe scoliosis in adult zebrafish. In the present study, we report for the first time the occurrence of but not of , in two tetrapod species of the genus. In , we show that mRNA-containing cells are CSF-contacting neurons. Furthermore, we identified the counterparts of zebrafish and we demonstrate that, as in zebrafish, it is expressed in the dorsal somatic musculature. Finally, we reveal that, in the disruption of results in an abnormal curvature of the antero-posterior axis of the tadpoles. Taken together, our results suggest that the role of the Utr4 signalling pathway in the control of body straightness is an ancestral feature of bony vertebrates and not just a peculiarity of ray-finned fishes.
Urp1 和 Urp2 是两种在硬骨鱼类中发现的脑肠肽家族的神经肽，主要在脑脊液（CSF）接触神经元中表达。最近有人提出，Urp1 和 Urp2 对于正确的轴形成和维持是必需的。它们的作用被认为是由受体 Uts2r3 介导的，Uts2r3 特异性地在背侧体节中表达。支持这一观点的是，已经证明缺失 会导致成年斑马鱼严重的脊柱侧凸。在本研究中，我们首次报道了在两个四足动物属的物种中存在 ，但不存在 。在 中，我们表明含有 mRNA 的细胞是 CSF 接触神经元。此外，我们鉴定了斑马鱼 的 对应物，并证明它与斑马鱼一样，在背侧体壁肌肉中表达。最后，我们揭示了在 中， 的破坏导致了蝌蚪前后轴的异常弯曲。总之，我们的结果表明，Utr4 信号通路在控制身体挺直方面的作用是骨脊椎动物的一个古老特征，而不仅仅是硬骨鱼类的一个特有特征。
