Rajanna M, Bharathi B, Shivakumar B R, Deepak M, Prashanth D'Souza, Prabakaran D, Vijayabhaskar T, Arun B
Surya Medical Centre, Bangalore, 560084, India.
Natural Remedies Pvt Ltd, Bangalore, 560100, India.
J Ayurveda Integr Med. 2021 Jul-Sep;12(3):529-534. doi: 10.1016/j.jaim.2021.06.004. Epub 2021 Aug 8.
Andrographis paniculata (Burm.f.) Nees has been well-researched for its immunomodulatory effects.
OBJECTIVE(S): To investigate the immunomodulatory effects of standardized A. paniculata extract (SAPE) in healthy adults.
The study was an open-label, single-centre study conducted for 30 days. Thirty participants with absolute lymphocyte counts of 1000-4000 cells/mm were enrolled and were instructed to ingest 200 mg of SAPE daily for 30 days. The participants visited the clinic at baseline, and days 3, 7, and 30. Immune cells such as NK cell (CD3-CD16+CD56+), T cells (CD3+), T helper cells (CD3+CD4+), T cytotoxic cells (CD3+CD8+) were measured using flow cytometry. Serum cytokines that include interferon gamma (IFN-γ), interleukin-4 (IL-4), interleukin-2 (IL-2), interleukin-12 (IL-12), and tumor necrosis factor alpha (TNF-α) were measured using ELISA. The SAPE used in this study was a standardized proprietary extract (AP-Bio®/KalmCold®) developed from the leaf extracts of A. paniculata.
SAPE increased T cells, T helper cells and significantly increased IFN-γ, IL-4, and decreased IL-2 at day 30. A subgroup analysis of participants with absolute lymphocyte counts of 1000-3000 cells/mm indicated that there is a significant increase in the T cells, T helper cells at day 7 and 30 and significant increase in IFN-γ, IL-4 and decrease in IL-2 at day 30. There was no treatment related adverse effects following SAPE intake for 30 days.
Supplementation of SAPE resulted in immunomodulatory effects evidenced by its effects on immune cells and cytokines and it was found to be safe and tolerable.
穿心莲已对其免疫调节作用进行了充分研究。
研究标准化穿心莲提取物（SAPE）对健康成年人的免疫调节作用。
本研究为开放标签、单中心研究，为期30天。招募了30名绝对淋巴细胞计数为1000 - 4000个细胞/mm³的参与者，并指示他们每天摄入200mg的SAPE，持续30天。参与者在基线以及第3、7和30天到诊所就诊。使用流式细胞术测量免疫细胞，如自然杀伤细胞（CD3⁻CD16⁺CD56⁺）、T细胞（CD3⁺）、辅助性T细胞（CD3⁺CD4⁺）、细胞毒性T细胞（CD3⁺CD8⁺）。使用酶联免疫吸附测定法测量血清细胞因子，包括干扰素γ（IFN - γ）、白细胞介素 - 4（IL - 4）、白细胞介素 - 2（IL - 2）、白细胞介素 - 12（IL - 12）和肿瘤坏死因子α（TNF - α）。本研究中使用的SAPE是一种从穿心莲叶提取物开发的标准化专利提取物（AP - Bio®/KalmCold®）。
在第30天，SAPE增加了T细胞、辅助性T细胞，并显著增加了IFN - γ、IL - 4，降低了IL - 2。对绝对淋巴细胞计数为1000 - 3000个细胞/mm³的参与者进行的亚组分析表明，在第7天和第30天T细胞、辅助性T细胞显著增加，在第30天IFN - γ、IL - 4显著增加，IL - 2降低。摄入SAPE 30天后未出现与治疗相关的不良反应。
补充SAPE导致了免疫调节作用，这通过其对免疫细胞和细胞因子的作用得到证明，并且发现它是安全且可耐受的。
