Xu Liguo, Li Wei, Sadeghi-Soureh Shima, Amirsaadat Soumaye, Pourpirali Raheleh, Alijani Sepideh
School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, China.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Biomed Mater Res A. 2022 Feb;110(2):316-330. doi: 10.1002/jbm.a.37288. Epub 2021 Aug 10.
Electrospun nanofibers (NFs)-based drug delivery approaches are of particular interest as a hopeful implantable nanoplatform for localized cancer therapy and treating tissue defect after resection, allowing the on-site drug delivery with minimal side effect to healthy cells. To maintain therapeutic concentrations of anticancer molecules for a relatively long time through a combination of burst and sustained drug release mechanisms, a hybrid of polycaprolactone and gelatin (PCL/GEL) was used for co-encapsulation of free curcumin (CUR) and CUR-loaded mesoporous silica nanoparticles (CUR@MSNs) via electrospinning, resulting in a novel drug-loaded nanofibrous scaffold, CUR/CUR@MSNs-NFs. The as-prepared MSNs and composite NFs were characterized via TGA, FTIR, FE-SEM, TEM, and BET. In vitro release profile of CUR from CUR/CUR@MSNs-NFs was examined, and the in vitro antitumor efficacy against MDA-MB-231 breast cancer cells was also evaluated through MTT, scratch assay, DAPI staining, and real-time PCR. The results disclosed that the smooth, bead-free, and randomly oriented CUR/CUR@MSNs-NFs displayed a combination of initial rapid discharge and sustained release for CUR, which led to higher cytotoxicity, lower migration as well as a more pronounced effect on apoptosis induction than CUR-NFs and CUR@MSNs-NFs. The present study illustrated that the dual drug release mechanisms through MSN/NF-mediated drug delivery systems might have a highly hopeful application as a localized implantable scaffold for potential postoperative breast cancer therapy.
基于电纺纳米纤维（NFs）的药物递送方法作为一种有前景的可植入纳米平台，用于局部癌症治疗和切除后组织缺损的治疗，引起了特别关注，它能够以最小的副作用向健康细胞进行原位药物递送。为了通过突释和持续释药机制的组合在较长时间内维持抗癌分子的治疗浓度，采用聚己内酯和明胶（PCL/GEL）的混合物通过静电纺丝共包封游离姜黄素（CUR）和负载CUR的介孔二氧化硅纳米颗粒（CUR@MSNs），从而得到一种新型的载药纳米纤维支架CUR/CUR@MSNs-NFs。通过热重分析（TGA）、傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FE-SEM）、透射电子显微镜（TEM）和比表面积分析仪（BET）对所制备的MSNs和复合NFs进行了表征。考察了CUR从CUR/CUR@MSNs-NFs中的体外释放曲线，并通过MTT法、划痕试验、4′,6-二脒基-2-苯基吲哚（DAPI）染色和实时聚合酶链反应（PCR）评估了其对MDA-MB-231乳腺癌细胞的体外抗肿瘤疗效。结果表明，光滑、无珠且随机取向的CUR/CUR@MSNs-NFs对CUR表现出初始快速释放和持续释放的组合，这导致其比CUR-NFs和CUR@MSNs-NFs具有更高的细胞毒性、更低的迁移率以及对细胞凋亡诱导更显著的作用。本研究表明，通过MSN/NF介导给药系统的双重释药机制作为一种潜在的术后乳腺癌治疗局部可植入支架可能具有非常有前景的应用。
