Department of Dental Biomaterials, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Maxillofacial Surgery, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Pharm Nanotechnol. 2023;11(4):364-372. doi: 10.2174/2211738511666230327123627.
This study aimed to prepare and evaluate the physicochemical and anticancer properties of cisplatin and curcumin-loaded mesoporous silica nanoparticles (Cis-Cur-MSNs).
In recent years, combination treatment has attained better outcomes than monotherapy in oncology. Cis-Cur-MSNs were prepared by precipitation technique.
The objective of the present study was the evaluation of the physicochemical and anticancer properties of cisplatin and curcumin-loaded mesoporous silica nanoparticles (Cis-Cur-MSNs).
The prepared materials were assessed in terms of physicochemical methods. The drug release pattern from the MSNs was also evaluated via ultraviolet spectrophotometry. In addition, the porosity and surface area of prepared nanoparticles were determined using the Brunauer-Emmett-Teller (BET) technique. The cytotoxicity of Cis-Cur-MSNs was evaluated on the HN5 cells as head and neck squamous carcinoma cell lines. Furthermore, ROS production of Cis-Cur-MSNs treated cells was evaluated compared with untreated cells.
According to the results, prepared nanoparticles displayed nanometer size, rod morphology, and negative surface charge with mesoporous structure belonging to the MCM-41 family (twodimensional hexagonal). Regarding the results of BET adsorption and desorption isotherm analysis for Cis-Cur-MSNs and drug-free MSNs, pore diameter, pore volume, specific surface area, and drug-loaded pore area in MSNs were decreased. In the first 10 days, the prepared nanoparticles exhibited a relatively rapid release pattern for cisplatin and curcumin, and until the 35th day, the release of them from the MSNs continued slowly.
The cytotoxic effect of Cis-Cur-MSNs was significantly more than Cur-MSNs and Cis- MSNs in 24 and 48 h incubation time (p < 0.05). The results suggest that Cis-Cur-MSNs may be beneficial in the development of a cancer treatment protocol. Others: The prepared nanoparticle in the present study could be a potential biomaterial for cancer treatment.
本研究旨在制备和评估顺铂和姜黄素负载介孔硅纳米粒子（Cis-Cur-MSNs）的理化性质和抗癌性能。
近年来，联合治疗在肿瘤学中比单一疗法取得了更好的效果。Cis-Cur-MSNs 是通过沉淀技术制备的。
本研究旨在评估顺铂和姜黄素负载介孔硅纳米粒子（Cis-Cur-MSNs）的理化性质和抗癌性能。
通过物理化学方法评估所制备材料。还通过紫外分光光度法评估 MSNs 中药物的释放模式。此外，还使用 Brunauer-Emmett-Teller（BET）技术测定了所制备纳米粒子的比表面积和孔隙率。采用头颈鳞癌细胞系 HN5 细胞评估 Cis-Cur-MSNs 的细胞毒性。此外，还评估了 Cis-Cur-MSNs 处理细胞与未处理细胞之间 ROS 的产生。
根据结果，所制备的纳米粒子呈纳米尺寸、棒状形态和负表面电荷，具有介孔结构，属于 MCM-41 家族（二维六方）。关于 Cis-Cur-MSNs 和无载药 MSNs 的 BET 吸附和解吸等温线分析结果，MSNs 的孔径、孔体积、比表面积和载药孔面积均减小。在最初的 10 天内，顺铂和姜黄素的释放模式相对较快，直到第 35 天，它们从 MSNs 中的释放仍在缓慢进行。
在 24 和 48 小时孵育时间内，Cis-Cur-MSNs 的细胞毒性作用明显强于 Cur-MSNs 和 Cis-MSNs（p<0.05）。结果表明，Cis-Cur-MSNs 可能有益于癌症治疗方案的制定。
本研究中制备的纳米粒子可能是癌症治疗的一种有前途的生物材料。
