Paul G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Molecular Engineering PhD Program, University of Washington, Seattle, WA, USA.
Nat Biotechnol. 2022 Jan;40(1):42-46. doi: 10.1038/s41587-021-01002-6. Epub 2021 Aug 12.
Detection of specific proteins using nanopores is currently challenging. To address this challenge, we developed a collection of over twenty nanopore-addressable protein tags engineered as reporters (NanoporeTERs, or NTERs). NTERs are constructed with a secretion tag, folded domain and a nanopore-targeting C-terminal tail in which arbitrary peptide barcodes can be encoded. We demonstrate simultaneous detection of up to nine NTERs expressed in bacterial or human cells using MinION nanopore sensor arrays.
目前，使用纳米孔检测特定蛋白质具有挑战性。为了解决这一挑战，我们开发了二十多种纳米孔可寻址的蛋白质标签，作为报告器（纳米孔靶向报告器，或 NTERs）。NTERs 由分泌标签、折叠结构域和一个纳米孔靶向 C 末端尾巴组成，其中可以编码任意肽条形码。我们使用 MinION 纳米孔传感器阵列，证明了在细菌或人类细胞中同时检测多达九个 NTER 的表达。
