Patankar Panini Shrikant, Joshi Shirish, Mane Abhishek, Manjesh P S, Kokate Dhananjay
Department of Pharmacology and Therapeutics, Seth GSMC and KEM Hospital, Mumbai, Maharashtra, India.
Tzu Chi Med J. 2021 Mar 15;33(3):263-269. doi: 10.4103/tcmj.tcmj_243_20. eCollection 2021 Jul-Sep.
The objective was to study the anxiolytic effect of minocycline in resident-intruder social conflict in submissive hamsters post resident intrusion model using open field test (OFT) and elevated plus maze (EPM) and serum cortisol levels.
Fifty-two singly housed male Syrian hamsters were used, post standardization of an animal model. Resident intrusion was done (5 min), in which smaller hamsters were placed in the cage of larger hamster, and the behavior of smaller hamster was noted. Eight submissive hamsters per group (disease control, lorazepam group as a positive control, and the test drug was minocycline) were used, and the drug was administered immediately post resident intrusion, intraperitoneally. Behavioral tests, namely OFT and EPM, were done followed by retro-orbital blood collection for serum cortisol estimation. The level of significance was set at < 0.05.
The minocycline group showed a statistically significant decrease in serum cortisol levels compared to the disease control group. Among all the variables pertaining to both the behavioral tests, namely EPM and OFT, the results indicated an anxiolytic effect, which was statistically significant compared to the disease control group.
As per the biochemical test using serum cortisol levels and behavioral tests in the form of EPM and OFT, the study concluded that the anxiolytic effect of minocycline is at least comparable to the positive control, lorazepam.
本研究旨在通过旷场试验（OFT）、高架十字迷宫（EPM）和血清皮质醇水平，探讨米诺环素对处于从属地位的仓鼠在定居者入侵模型中定居者 - 入侵者社会冲突的抗焦虑作用。
使用52只单笼饲养的雄性叙利亚仓鼠，在动物模型标准化后，进行定居者入侵（5分钟），即将较小的仓鼠放入较大仓鼠的笼中，并记录较小仓鼠的行为。每组使用8只从属仓鼠（疾病对照组、作为阳性对照的劳拉西泮组以及受试药物米诺环素组），在定居者入侵后立即腹腔注射给药。进行行为测试，即旷场试验和高架十字迷宫试验，然后通过眶后采血来估计血清皮质醇水平。显著性水平设定为<0.05。
与疾病对照组相比，米诺环素组血清皮质醇水平有统计学意义的降低。在与两种行为测试（即高架十字迷宫试验和旷场试验）相关的所有变量中，结果表明具有抗焦虑作用，与疾病对照组相比具有统计学意义。
根据使用血清皮质醇水平的生化测试以及高架十字迷宫试验和旷场试验形式的行为测试，该研究得出结论，米诺环素的抗焦虑作用至少与阳性对照劳拉西泮相当。
