从云南重楼中分离到的新型 Tombusviridae 病毒的鉴定。
Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis.
机构信息
Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.
Research and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 495 Jinhei Road, Kunming, 650201, Yunnan, China.
出版信息
Arch Virol. 2021 Nov;166(11):3199-3205. doi: 10.1007/s00705-021-05191-y. Epub 2021 Aug 13.
A novel virus, Paris virus 2 (ParV2), was isolated from diseased Paris polyphylla var. yunnanensis, and its complete genome sequence was determined and analyzed. ParV2 is a positive-sense single-stranded RNA (+ssRNA) virus with a genome size of 4,118 nucleotides. The ParV2 genome contains six putative open reading frames (ORFs) that encode proteins with predicted molecular weights of 40.14, 100.26, 7.31, 7.85, 26.09, and 8.77 kDa. The first ORF (ORF1) of ParV2 encodes a putative protein of 40.14 kDa (P40, nt: 20-1,096), whiles the second ORF (ORF2, 888 aa) containing the GDD motif encodes the highly conserved RNA-dependent RNA polymerase protein (RdRP, nt:20-2,683, P100, 100.26 kDa) of viruses in the family Tombusviridae. Multiple sequence alignments analysis showed that the complete genome sequence of ParV2 shares 31.7-55.5% nucleotide sequence identities with viruses in the family Tombusviridae. Ginger chlorotic fleck-associated tombusvirus (GCFaV-1, Accession No. QKE30557) had the highest sequence identity (55.5%) with ParV2. GCFaV-1 also shares 59.2% RdRP and 34.9% CP amino acid sequence identities with ParV2. Sequence comparisons and phylogenetic analysis of RdRP suggested that ParV2 is a novel member of the family Tombusviridae, and its closest known relative is GCFaV-1.
一种新型病毒，巴黎病毒 2（ParV2），从患病的云南重楼中分离出来，并确定和分析了其完整的基因组序列。ParV2 是一种正链单链 RNA（+ssRNA）病毒，基因组大小为 4118 个核苷酸。ParV2 基因组包含六个推定的开放阅读框（ORF），编码预测分子量为 40.14、100.26、7.31、7.85、26.09 和 8.77 kDa 的蛋白质。ParV2 的第一个 ORF（ORF1）编码一个 40.14 kDa 的推定蛋白（P40，nt：20-1096），而包含 GDD 基序的第二个 ORF（ORF2，888 aa）编码高度保守的病毒 RNA 依赖性 RNA 聚合酶蛋白（RdRP，nt：20-2683，P100，100.26 kDa）。家族 Tombusviridae 的病毒。多个序列比对分析表明，ParV2 的完整基因组序列与家族 Tombusviridae 的病毒具有 31.7-55.5%的核苷酸序列同一性。姜黄花斑相关番茄丛矮病毒（GCFaV-1，登录号 QKE30557）与 ParV2 的序列同一性最高（55.5%）。GCFaV-1 与 ParV2 的 RdRP 也具有 59.2%和 CP 氨基酸序列 34.9%的同一性。RdRP 的序列比较和系统发育分析表明，ParV2 是家族 Tombusviridae 的一个新成员，其最接近的已知亲缘关系是 GCFaV-1。
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