从滇重楼分离出的一种新型马铃薯Y病毒的全基因组序列分析
Complete genome sequence analysis of a new potyvirus isolated from Paris polyphylla var. yunnanensis.
作者信息
Zhang Boxin, Li Qiannan, Hu Jingyu, Zhang Lei, Dong Xian, Ji Pengzhang, Dong Jiahong
机构信息
School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Yunnan, 650500, China.
Institute of Medicinal Plant Cultivation, Academy of Southern Medicine, Yunnan University of Chinese Medicine, Yunnan, 650500, China.
出版信息
Arch Virol. 2023 Jan 7;168(2):43. doi: 10.1007/s00705-022-05655-9.
The complete genome sequence of a new potyvirus from Paris polyphylla var. yunnanensis was determined. Its genomic RNA consists of 9571 nucleotides (nt), excluding the 3'-terminal poly(A) tail, containing the typical open reading frame (ORF) of potyviruses and encoding a putative large polyprotein of 3061 amino acids. The virus shares 54.20%-59.60% nt sequence identity and 51.80%-57.90% amino acid sequence identity with other potyviruses. Proteolytic cleavage sites and conserved motifs of potyviruses were identified in the polyprotein and within individual proteins. Phylogenetic analysis indicated that the virus was most closely related to lily yellow mosaic virus. The results suggest that the virus should be classified as a member of a novel species within the genus Potyvirus, and we have tentatively named this virus "Paris yunnanensis mosaic chlorotic virus" (PyMCV).
测定了来自滇重楼的一种新型马铃薯Y病毒的全基因组序列。其基因组RNA由9571个核苷酸（nt）组成，不包括3'-末端的聚腺苷酸尾，含有马铃薯Y病毒典型的开放阅读框（ORF），并编码一个推定的由3061个氨基酸组成的大的多聚蛋白。该病毒与其他马铃薯Y病毒的核苷酸序列同一性为54.20%-59.60%，氨基酸序列同一性为51.80%-57.90%。在多聚蛋白和各个蛋白质中鉴定出了马铃薯Y病毒的蛋白水解切割位点和保守基序。系统发育分析表明，该病毒与百合黄花叶病毒关系最为密切。结果表明，该病毒应被归类为马铃薯Y病毒属内一个新物种的成员，我们暂定将该病毒命名为“滇重楼花叶褪绿病毒”（PyMCV）。
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