Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.
Research and Development Center for Heath Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 32 Jiaochang Donglu, Kunming, 650201, Yunnan, China.
Arch Virol. 2020 Apr;165(4):985-988. doi: 10.1007/s00705-020-04560-3. Epub 2020 Feb 25.
The complete genome sequence of a novel potyvirus, tentatively named "paris virus 1" (ParV1, GenBank accession no. MN549985), infecting Paris polyphylla var. yunnanensis was determined in this study. A single large open reading frame (nt 96-9818) encoding a 3240-aa polyprotein that is predicted to be cleaved into 10 mature proteins was detected in the ParV1 genome. Comparative analysis of the ParV1 genome sequence with those of other potyviruses identified nine cleavage sites and conserved motifs that are typical features of potyviruses. Pairwise sequence comparisons showed that the ParV1 polyprotein shares 49.6-65.1% nucleotide and 47.1-68.9% amino acid sequence identity with viruses of the genus Potyvirus. BLAST analysis revealed that ParV1 shares 65.1% nucleotide and 68.9% amino acid sequence identity with Thunberg fritillary mosaic virus (TFMV, accession no. CAI59123), its closest known relative. These results suggest that paris virus 1 (ParV1) is a new member of the genus Potyvirus.
本研究测定了一种感染云南重楼的新型马铃薯 Y 病毒（暂定名为“巴黎病毒 1”，ParV1，GenBank 登录号 MN549985）的全基因组序列。ParV1 基因组中检测到一个单一的大开放阅读框（nt 96-9818），编码一个预测被切割成 10 个成熟蛋白的 3240 个氨基酸的多蛋白。ParV1 基因组序列与其他马铃薯 Y 病毒的比较分析确定了 9 个切割位点和保守基序，这些是马铃薯 Y 病毒的典型特征。序列比对表明，ParV1 多蛋白与属内病毒的核苷酸和氨基酸序列同一性分别为 49.6-65.1%和 47.1-68.9%。BLAST 分析显示，ParV1 与最接近的已知亲缘病毒——延龄草花叶病毒（TFMV，登录号 CAI59123）的核苷酸和氨基酸序列同一性分别为 65.1%和 68.9%。这些结果表明，巴黎病毒 1（ParV1）是马铃薯 Y 病毒属的一个新成员。
