University of Fribourg, Department of Chemistry, chemin du musée 9, Fribourg 1700, Switzerland.
Chem Commun (Camb). 2021 Sep 11;57(70):8726-8729. doi: 10.1039/d1cc02597j. Epub 2021 Aug 16.
SilE and SilB are both proteins involved in the silver efflux pump found in Gram-negative bacteria such as S. typhimurium. Using model peptides along with NMR and CD experiments, we show how SilE may store silver ions prior to delivery and we hypothesize for the first time the interplay between SilB and SilE.
SilE 和 SilB 都是革兰氏阴性菌(如鼠伤寒沙门氏菌)中的银外排泵所涉及的蛋白质。通过使用模型肽以及 NMR 和 CD 实验,我们展示了 SilE 如何在输送之前储存银离子,并且我们首次假设了 SilB 和 SilE 之间的相互作用。