Rao Dan, Zheng Shi-Gang, Zhao Ting-Mei, Yan Shou, Hu Ya-Dong, Zhao Ruo-Xi, Li Hong-Jie, Wang Qin, Gu Liang-Yun, Chun Ze
Chengdu Institute of Biology,Chinese Academy of Sciences,CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu 610041,China College of Life Science,University of Chinese Academy of Sciences Beijing 100049,China.
Chengdu Institute of Biology,Chinese Academy of Sciences,CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu 610041,China.
Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(13):3330-3336. doi: 10.19540/j.cnki.cjcmm.20210320.102.
The present study aimed to explore the correlation between agronomic traits and quality indexes of Dendrobium nobile and its application value in agricultural breeding. The cultivated strains of D. nobile in Hejiang-Chishui producing areas were extensively collected,and the main agronomic traits and quality indexes were measured. The agronomic traits with significant correlation with quality indexes were screened out by the correlation analysis,and then the parental lines and self-bred F_1 generation plants were furtherverified. Among 96 lines of D. nobile,the content of soluble polysaccharides showed a significant negative correlation with dendrobine( P < 0. 01),and no significant correlation with agronomic traits in stems and leaves. The content of dendrobine exhibited a significant positive correlation with the stem width-thickness ratio( at the largest cross section; P < 0. 01),and no significant correlation with other agronomic traits. Regression analysis further verified the positive correlation between dendrobine content and stem width-thickness ratio( R2> 0. 9). Two lines,JC-10 and JC-35,with significant differences in stem width-thickness ratio were screened out( P <0. 05). The corresponding F1 generation plants by self-pollination both showed that the dendrobine content was higher with greater stem width-thickness ratio( P < 0. 01). The experimental results suggested that within a certain range,the dendrobine content was higher in D. nobile with flatter stem. Therefore,in the breeding of D. nobile,this specific trait could be used for screening plants with high content of quality indexes such as dendrobine.
本研究旨在探讨铁皮石斛农艺性状与品质指标之间的相关性及其在农业育种中的应用价值。广泛收集了合江 - 赤水产区的栽培铁皮石斛菌株，测定了主要农艺性状和品质指标。通过相关性分析筛选出与品质指标显著相关的农艺性状，然后对亲本系和自交F_1代植株进行进一步验证。在96个铁皮石斛株系中，可溶性多糖含量与石斛碱呈显著负相关（P < 0.01），与茎、叶农艺性状无显著相关性。石斛碱含量与茎宽厚比（最大横切面处；P < 0.01）呈显著正相关，与其他农艺性状无显著相关性。回归分析进一步验证了石斛碱含量与茎宽厚比之间的正相关（R2>0.9）。筛选出两个茎宽厚比差异显著的株系JC - 10和JC - 35（P <0.05）。通过自花授粉得到的相应F1代植株均表明，茎宽厚比越大，石斛碱含量越高（P < 0.01）。实验结果表明，在一定范围内，茎越扁平的铁皮石斛中石斛碱含量越高。因此，在铁皮石斛育种中，这一特定性状可用于筛选石斛碱等品质指标含量高的植株。
