Zhao Yongxia, Ji Xiaolong, Liu Xiaoqi, Qin Lin, Tan Daopeng, Wu Di, Bai Chaojun, Yang Jiyong, Xie Jian, He Yuqi
Guizhou Engineering Research Center of Industrial Key-Technology for Dendrobium nobile, Engineering Research Center of Pharmaceutical Orchid Plant Breeding, High Efficiency Application in Guizhou Province, Zunyi Medical University, Zunyi, China.
Key Laboratory of Basic Pharmacology of Ministry of Education, Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, China.
Front Microbiol. 2023 Dec 8;14:1294402. doi: 10.3389/fmicb.2023.1294402. eCollection 2023.
, a valued Chinese herb known for its diverse pharmacological effects, owes much of its potency to the bioactive compound dendrobine. However, dendrobine content varies significantly with plant age, and the mechanisms governing this variation remain unclear. This study delves into the potential role of endophytic fungi in shaping host-microbe interactions and influencing plant metabolism.
Using RNA-seq, we examined the transcriptomes of 1-year-old, 2-year-old, and 3-year-old samples and through a comprehensive analysis of endophytic fungal communities and host gene expression in stems of varying ages, we aim to identify associations between specific fungal taxa and host genes.
The results revealing 192 differentially expressed host genes. These genes exhibited a gradual decrease in expression levels as the plants aged, mirroring dendrobine content changes. They were enriched in 32 biological pathways, including phagosome, fatty acid degradation, alpha-linolenic acid metabolism, and plant hormone signal transduction. Furthermore, a significant shift in the composition of the fungal community within stems was observed along the age gradient. , , and dominated in 1-year-old plants, while and prevailed in 2-year-old plants. Conversely, 3-year-old plants exhibited additional enrichment of endophytic fungi, including the genus . Two gene expression modules (mediumpurple3 and darkorange) correlated significantly with dominant endophytic fungi abundance and dendrobine accumulation. Key genes involved in dendrobine synthesis were found associated with plant hormone synthesis.
This study suggests that the interplay between different endophytic fungi and the hormone signaling system in likely regulates dendrobine biosynthesis, with specific endophytes potentially triggering hormone signaling cascades that ultimately influence dendrobine synthesis.
是一种珍贵的中草药，以其多样的药理作用而闻名，其药效很大程度上归功于生物活性化合物石斛碱。然而，石斛碱含量随植株年龄变化显著，而控制这种变化的机制仍不清楚。本研究深入探讨内生真菌在塑造宿主 - 微生物相互作用和影响植物代谢方面的潜在作用。
我们使用RNA测序技术检测了1年生、2年生和3年生 样本的转录组，并通过对不同年龄 茎中内生真菌群落和宿主基因表达的综合分析，旨在确定特定真菌类群与宿主基因之间的关联。
结果揭示了192个差异表达的宿主基因。随着植株年龄增长，这些基因的表达水平逐渐下降，这与石斛碱含量变化一致。它们富集于32条生物学途径，包括吞噬体、脂肪酸降解、α-亚麻酸代谢和植物激素信号转导。此外，沿着年龄梯度观察到 茎内真菌群落组成发生了显著变化。 、 和 在1年生植株中占主导地位，而 和 在2年生植株中占优势。相反，3年生植株表现出内生真菌的额外富集，包括 属。两个基因表达模块（中紫色3和深橙色）与优势内生真菌丰度和石斛碱积累显著相关。发现参与石斛碱合成的关键基因与植物激素合成有关。
本研究表明， 中不同内生真菌与激素信号系统之间的相互作用可能调节石斛碱生物合成，特定内生菌可能触发激素信号级联反应，最终影响石斛碱合成。
